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dc.date.accessioned2017-02-23T11:57:43Z
dc.date.available2017-02-23T11:57:43Z
dc.date.created2017-01-13T14:41:11Z
dc.date.issued2016
dc.identifier.citationHøydahl, Lene Støkken Nilssen, Nicolay Rustad Gunnarsen, Kristin Støen du Pré, Marie Fleur Iversen, Rasmus Roos, Norbert Chen, Xi Michaelsen, Terje Einar Sollid, Ludvig Magne Sandlie, Inger Løset, Geir Åge . Multivalent pIX phage display selects for distinct and improved antibody properties. Scientific Reports. 2016, 6
dc.identifier.urihttp://hdl.handle.net/10852/54086
dc.description.abstractPhage display screening readily allows for the identification of a multitude of antibody specificities, but to identify optimal lead candidates remains a challenge. Here, we direct the antibody-capsid fusion away from the signal sequence-dependent secretory SEC pathway in E. coli by utilizing the intrinsic signal sequence-independent property of pIX to obtain virion integration. This approach was combined with the use of an engineered helper phage known to improve antibody pIX display and retrieval. By direct comparison with pIII display, we demonstrate that antibody display using this pIX system translates into substantially improved retrieval of desired specificities with favorable biophysical properties in de novo selection. We show that the effect was due to less E. coli host toxicity during phage propagation conferred by the lack of a signal sequence. This pIX combinatorial display platform provides a generic alternative route for obtaining good binders with high stability and may thus find broad applicability.en_US
dc.languageEN
dc.language.isoenen_US
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleMultivalent pIX phage display selects for distinct and improved antibody propertiesen_US
dc.typeJournal articleen_US
dc.creator.authorHøydahl, Lene Støkken
dc.creator.authorNilssen, Nicolay Rustad
dc.creator.authorGunnarsen, Kristin Støen
dc.creator.authordu Pré, Marie Fleur
dc.creator.authorIversen, Rasmus
dc.creator.authorRoos, Norbert
dc.creator.authorChen, Xi
dc.creator.authorMichaelsen, Terje Einar
dc.creator.authorSollid, Ludvig Magne
dc.creator.authorSandlie, Inger
dc.creator.authorLøset, Geir Åge
cristin.unitcode185,53,18,12
cristin.unitnameAvdeling for immunologi og transfusjonsmedisin
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.fulltextoriginal
cristin.qualitycode1
dc.identifier.cristin1426899
dc.identifier.bibliographiccitationinfo:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Scientific Reports&rft.volume=6&rft.spage=&rft.date=2016
dc.identifier.jtitleScientific Reports
dc.identifier.volume6
dc.identifier.pagecount13
dc.identifier.doihttp://dx.doi.org/10.1038/srep39066
dc.identifier.urnURN:NBN:no-57213
dc.type.documentTidsskriftartikkelen_US
dc.type.peerreviewedPeer reviewed
dc.source.issn2045-2322
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/54086/1/srep39066.pdf
dc.type.versionPublishedVersion
cristin.articleid39066


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