dc.date.accessioned | 2017-02-23T11:57:43Z | |
dc.date.available | 2017-02-23T11:57:43Z | |
dc.date.created | 2017-01-13T14:41:11Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Høydahl, Lene Støkken Nilssen, Nicolay Rustad Gunnarsen, Kristin Støen du Pré, Marie Fleur Iversen, Rasmus Roos, Norbert Chen, Xi Michaelsen, Terje Einar Sollid, Ludvig Magne Sandlie, Inger Løset, Geir Åge . Multivalent pIX phage display selects for distinct and improved antibody properties. Scientific Reports. 2016, 6 | |
dc.identifier.uri | http://hdl.handle.net/10852/54086 | |
dc.description.abstract | Phage display screening readily allows for the identification of a multitude of antibody specificities, but to identify optimal lead candidates remains a challenge. Here, we direct the antibody-capsid fusion away from the signal sequence-dependent secretory SEC pathway in E. coli by utilizing the intrinsic signal sequence-independent property of pIX to obtain virion integration. This approach was combined with the use of an engineered helper phage known to improve antibody pIX display and retrieval. By direct comparison with pIII display, we demonstrate that antibody display using this pIX system translates into substantially improved retrieval of desired specificities with favorable biophysical properties in de novo selection. We show that the effect was due to less E. coli host toxicity during phage propagation conferred by the lack of a signal sequence. This pIX combinatorial display platform provides a generic alternative route for obtaining good binders with high stability and may thus find broad applicability. | en_US |
dc.language | EN | |
dc.language.iso | en | en_US |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | Multivalent pIX phage display selects for distinct and improved antibody properties | en_US |
dc.type | Journal article | en_US |
dc.creator.author | Høydahl, Lene Støkken | |
dc.creator.author | Nilssen, Nicolay Rustad | |
dc.creator.author | Gunnarsen, Kristin Støen | |
dc.creator.author | du Pré, Marie Fleur | |
dc.creator.author | Iversen, Rasmus | |
dc.creator.author | Roos, Norbert | |
dc.creator.author | Chen, Xi | |
dc.creator.author | Michaelsen, Terje Einar | |
dc.creator.author | Sollid, Ludvig Magne | |
dc.creator.author | Sandlie, Inger | |
dc.creator.author | Løset, Geir Åge | |
cristin.unitcode | 185,53,18,12 | |
cristin.unitname | Avdeling for immunologi og transfusjonsmedisin | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 1426899 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Scientific Reports&rft.volume=6&rft.spage=&rft.date=2016 | |
dc.identifier.jtitle | Scientific Reports | |
dc.identifier.volume | 6 | |
dc.identifier.pagecount | 13 | |
dc.identifier.doi | http://dx.doi.org/10.1038/srep39066 | |
dc.identifier.urn | URN:NBN:no-57213 | |
dc.type.document | Tidsskriftartikkel | en_US |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 2045-2322 | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/54086/1/srep39066.pdf | |
dc.type.version | PublishedVersion | |
cristin.articleid | 39066 | |