Unveiling phenotypic differences among the genetically conserved Mycobacterium tuberculosis complex: Characterization of proteomes and post-translational modifications
Metadata
Vis metadataFinnes i følgende samling
- Institutt for klinisk medisin [10751]
- CRIStin høstingsarkiv [31257]
Sammendrag
Members of the genetically conserved Mycobacterium tuberculosis complex (MTBC) exhibit vast discrepancies in clinical outcome and epidemiological behaviour. Bacterial factors that contribute to these phenotypic variabilities remain elusive. We hypothesize that qualitative and quantitative differences in the abundance of proteins and their post-translational modifications (PTMs) may contribute to MTBC phenotypic diversity. The study analyzed the proteomic and PTM profiles (acetylation and glycosylation) among members of the MTBC using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Comparative proteomic analysis of the MTBC lineages revealed significantly different abundances of proteins involved in virulence, growth and bioenergetics. The PTM study provides the first report on O-acetylation and N-glycosylation of MTBC proteins. The study presents a comprehensive proteomic and PTM map of MTBC and demonstrated the presence of significant differences among different lineages. These new discoveries will enable a better understanding of the pathogen with new potential for identification of novel drug targets and vaccine candidates, as well as efficient diagnostics for TB disease.Artikkelliste
Paper I. Yimer SA, Birhanu AG, Kalayou S, Riaz T, Zegeye ED, Beyene GT, Holm-Hansen C, Norheim G, Abebe M, Aseffa A, Tønjum T. Comparative Proteomic Analysis of Mycobacterium tuberculosis Lineage 7 and Lineage 4 Strains Reveals Differentially Abundant Proteins Linked to Slow Growth and Virulence. Frontiers in Microbiology. 2017;8. DOI:10.3389/fmicb.2017.00795. The paper is included in the thesis. Also available at: https://doi.org/10.3389/fmicb.2017.00795 |
Paper II. Birhanu AG, Yimer SA, Holm-Hansen C, Norheim G, Aseffa A, Abebe M, Tønjum T. Nε-and O-Acetylation in Mycobacterium tuberculosis lineage 7 and lineage 4 Strains: Proteins Involved in Bioenergetics, Virulence, and Antimicrobial Resistance Are Acetylated. Journal of proteome research. 2017 Oct 4;16(11):4045-59. DOI:10.1021/acs.jproteome.7b00429. The article is not available in DUO due to publisher restrictions. The accepted version is available in DUO: http://urn.nb.no/URN:NBN:no-69454 |
Paper III. Birhanu AG, Yimer SA, Kalayou S, Riaz T, Zegeye ED, Holm-Hansen C, Norheim G, Aseffa A, Abebe M, Tønjum T. Ample glycosylation in membrane and cell envelope proteins may explain the phenotypic diversity and virulence in the Mycobacterium tuberculosis complex. Scientific Reports 2019, 9(1):2927. DOI:10.1038/s41598-019-39654-9. The paper is included in the thesis. Also available in DUO: http://urn.nb.no/URN:NBN:no-77471 |