dc.date.accessioned | 2015-11-25T12:00:58Z | |
dc.date.available | 2015-11-25T12:00:58Z | |
dc.date.created | 2015-07-27T22:58:17Z | |
dc.date.issued | 2015 | |
dc.identifier.citation | Bengtsen, Mads Klepper, Kjetil Gundersen, Sveinung Cuervo, Ignacio Drabløs, Finn Hovig, Johannes Eivind Sandve, Geir Kjetil F. Gabrielsen, Odd Stokke Eskeland, Ragnhild . c-Myb Binding Sites in Haematopoietic Chromatin Landscapes. PLoS ONE. 2015, 10(7) | |
dc.identifier.uri | http://hdl.handle.net/10852/47870 | |
dc.description.abstract | Strict control of tissue-specific gene expression plays a pivotal role during lineage commit- ment. The transcription factor c-Myb has an essential role in adult haematopoiesis and func- tions as an oncogene when rearranged in human cancers. Here we have exploited digital genomic footprinting analysis to obtain a global picture of c-Myb occupancy in the genome of six different haematopoietic cell-types. We have biologically validated several c-Myb foot- prints using c-Myb knockdown data, reporter assays and DamID analysis. We show that our predicted conserved c-Myb footprints are highly dependent on the haematopoietic cell type, but that there is a group of gene targets common to all cell-types analysed. Further- more, we find that c-Myb footprints co-localise with active histone mark H3K4me3 and are significantly enriched at exons. We analysed co-localisation of c-Myb footprints with 104 chromatin regulatory factors in K562 cells, and identified nine proteins that are enriched together with c-Myb footprints on genes positively regulated by c-Myb and one protein enriched on negatively regulated genes. Our data suggest that c-Myb is a transcription fac- tor with multifaceted target regulation depending on cell type. | en_US |
dc.language | EN | |
dc.language.iso | en | en_US |
dc.publisher | Public Library of Science (PLoS) | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.title | c-Myb Binding Sites in Haematopoietic Chromatin Landscapes | en_US |
dc.type | Journal article | en_US |
dc.creator.author | Bengtsen, Mads | |
dc.creator.author | Klepper, Kjetil | |
dc.creator.author | Gundersen, Sveinung | |
dc.creator.author | Cuervo, Ignacio | |
dc.creator.author | Drabløs, Finn | |
dc.creator.author | Hovig, Johannes Eivind | |
dc.creator.author | Sandve, Geir Kjetil F. | |
dc.creator.author | Gabrielsen, Odd Stokke | |
dc.creator.author | Eskeland, Ragnhild | |
cristin.unitcode | 185,15,29,40 | |
cristin.unitname | Seksjon for biokjemi og molekylærbiologi | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.cristin | 1255287 | |
dc.identifier.bibliographiccitation | info:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=PLoS ONE&rft.volume=10&rft.spage=&rft.date=2015 | |
dc.identifier.jtitle | PLoS ONE | |
dc.identifier.volume | 10 | |
dc.identifier.issue | 7 | |
dc.identifier.doi | http://dx.doi.org/10.1371/journal.pone.0133280 | |
dc.identifier.urn | URN:NBN:no-51894 | |
dc.type.document | Tidsskriftartikkel | en_US |
dc.type.peerreviewed | Peer reviewed | |
dc.source.issn | 1932-6203 | |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/47870/2/fetchObject98788.pdf | |
dc.type.version | PublishedVersion | |
cristin.articleid | e0133280 | |