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A high-throughput pipeline for validation of antibodies

dc.date.accessioned2019-04-08T10:29:23Z
dc.date.available2019-04-08T10:29:23Z
dc.date.created2018-12-13T12:25:23Z
dc.date.issued2018
dc.identifier.citationSikorski, Krzysztof Mehta, Adi Inngjerdingen, Marit Thakor, Flourina Kumar Kling, Simon Kalina, Tomas Nyman, Tuula Anneli Stensland, Maria Zhou, Wei De Souza, Gustavo Antonio Holden, Lars Stuchly, Jan Templin, Markus Lund-Johansen, Fridtjof . A high-throughput pipeline for validation of antibodies. Nature Methods. 2018, 15(11), 909-912
dc.identifier.urihttp://hdl.handle.net/10852/67592
dc.description.abstractWestern blotting (WB) is widely used to test antibody specificity, but the assay has low throughput and precision. Here we used preparative gel electrophoresis to develop a capture format for WB. Fractions with soluble, size-separated proteins facilitated parallel readout with antibody arrays, shotgun mass spectrometry (MS) and immunoprecipitation followed by MS (IP-MS). This pipeline provided the means for large-scale implementation of antibody validation concepts proposed by an international working group on antibody validation (IWGAV).Western blotting (WB) is widely used to test antibody specificity, but the assay has low throughput and precision. Here we used preparative gel electrophoresis to develop a capture format for WB. Fractions with soluble, size-separated proteins facilitated parallel readout with antibody arrays, shotgun mass spectrometry (MS) and immunoprecipitation followed by MS (IP-MS). This pipeline provided the means for large-scale implementation of antibody validation concepts proposed by an international working group on antibody validation (IWGAV).
dc.description.abstractA high-throughput pipeline for validation of antibodies
dc.languageEN
dc.publisherNature Publishing Group
dc.titleA high-throughput pipeline for validation of antibodies
dc.typeJournal article
dc.creator.authorSikorski, Krzysztof
dc.creator.authorMehta, Adi
dc.creator.authorInngjerdingen, Marit
dc.creator.authorThakor, Flourina Kumar
dc.creator.authorKling, Simon
dc.creator.authorKalina, Tomas
dc.creator.authorNyman, Tuula Anneli
dc.creator.authorStensland, Maria
dc.creator.authorZhou, Wei
dc.creator.authorDe Souza, Gustavo Antonio
dc.creator.authorHolden, Lars
dc.creator.authorStuchly, Jan
dc.creator.authorTemplin, Markus
dc.creator.authorLund-Johansen, Fridtjof
cristin.unitcode185,53,49,70
cristin.unitnameK. G. Jebsen senter for immunterapi mot kreft - part UiO
cristin.ispublishedtrue
cristin.fulltextpostprint
cristin.qualitycode2
dc.identifier.cristin1642639
dc.identifier.bibliographiccitationinfo:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Nature Methods&rft.volume=15&rft.spage=909&rft.date=2018
dc.identifier.jtitleNature Methods
dc.identifier.volume15
dc.identifier.issue11
dc.identifier.startpage909
dc.identifier.endpage912
dc.identifier.doihttp://dx.doi.org/10.1038/s41592-018-0179-8
dc.identifier.urnURN:NBN:no-70766
dc.type.documentTidsskriftartikkel
dc.type.peerreviewedPeer reviewed
dc.source.issn1548-7091
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/67592/1/An%2Bantibody%2Barray%2Bwestern%2Bblot.pdf
dc.type.versionAcceptedVersion
dc.relation.projectNFR/237718


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