In silico analyzes of porins involved in niche adaptation: Exploring the role of Helicobacter pylori outer membrane phospholipase A in acid tolerance.
AbstractHelicobacter pylori is an ulcer-causing bacterium that evolutionary needs to adapt quickly to changing conditions. Hilde Synnøve Vollan’s bioinformatics studies revealed that H. pylori outer membrane phospholipase A (OMPLA) is multifunctional with both enzymatic and pore activity. OMPLA is a protein found in a variety of bacterial species, and usually functions as an enzyme that degrades the outer membrane. Previous studies indicate that OMPLA must be intact for H. pylori survival in an acidic environment, while its enzymatic activity is not required. The present study presents the hypothesis that OMPLA is involved in acid management. Analyzes of the modeled H. pylori OMPLA 3D structure indicate that the niche-adaption likely is urea-influx and ammonium-efflux. This model may be paradigmatic for all gastric Helicobacter species. This work will be used in future studies aimed at preventing ulcers and gastric cancers caused by H. pylori.
List of papers
|Paper I: In Silico Structure and Sequence Analysis of Bacterial Porins and Specific Diffusion Channels for Hydrophilic Molecules: Conservation, Multimericity and Multifunctionality. Vollan HS, Tannæs T, Vriend G, Bukholm G. Int J Mol Sci. 2016 Apr 21;17(4). doi: 10.3390/ijms17040599. The paper is available in DUO: http://hdl.handle.net/10852/52459|
|Paper II: In silico evolutionary analysis of Helicobacter pylori outer membrane phospholipase A (OMPLA). Vollan HS, Tannaes T, Yamaoka Y, Bukholm G. BMC Microbiol. 2012 Sep 13;12:206. doi: 10.1186/1471-2180-12-206. The paper is available in DUO: http://hdl.handle.net/10852/47119|
|Paper III: Outer membrane phospholipase A's roles in Helicobacter pylori acid adaptation. Vollan HS, Tannæs T, Caugant DA, Vriend G, Bukholm G. Gut Pathog. 2017 Jun 12;9:36. doi: 10.1186/s13099-017-0184-y. eCollection 2017. The paper is available in DUO: http://hdl.handle.net/10852/55661|