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Now showing items 11-20 of 23
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2021)
This paper describes smart sampling paper to be used for bottom-up protein analysis. Four different manners to immobilize trypsin on cellulose were evaluated. Untreated paper, potassium-periodate-functionalized paper (with ...
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2021)
In the present work, a pair of molecularly imprinted polymers (MIPs) targeting distinct peptide targets were packed into trap columns and combined for automated duplex analysis of two low abundant small cell lung cancer ...
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2021)
Antibody-based affinity capture has become the gold standard in sample preparation for determination of low-abundance protein biomarkers in biological matrices prior to liquid chromatography-mass spectrometry (LC-MS) ...
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2021)
This initial study evaluates vacuum matrix-assisted ionization (vMAI) mass spectrometry (MS) for identification and determination of tryptic peptides from the biomarker protein progastrin releasing peptide (ProGRP). Similar ...
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2021)
For the first time, we report electromembrane extraction (EME) of peptides using deep eutectic solvent (DES) as supported liquid membrane (SLM). DES were mixtures of coumarin, camphor, DL-menthol and thymol. Sixteen model ...
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2022)
Abstract The modification of an easily available resource like paper to circumvent expensive or intensive sample pretreatment could be the answer to sample analysis in resource-poor regions. Therefore, a novel on-paper ...
The utility of molecularly imprinted polymers for mass spectrometric protein and proteomics analysis
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2022)
Selective and efficient sample clean-up is important in mass spectrometric protein- and proteomics analyses from biological matrices. Molecularly imprinted polymers (MIPs), polymers prepared to have tailor-made cavities ...
(Journal article / Tidsskriftartikkel / AcceptedVersion; Peer reviewed, 2022)
Here the relation between digestion of proteins by trypsin covalently bound to paper and trypsin in-solution is investigated. The trypsin acting on paper is covalently bound. A trypsin concentration of 0.5% (w/v) results ...
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2022)
The purpose of this study was to develop a smart proteolysis sampler based on commercially available VAMS® tips employing Volumetric Absorptive Microsampling. In this proof-of-concept paper we show that covalently bound ...
(Journal article / Tidsskriftartikkel / PublishedVersion; Peer reviewed, 2019)
Immunocapture in mass spectrometry based targeted protein analysis using a bottom-up workflow is nowadays mainly performed by target protein extraction using anti-protein antibodies followed by tryptic digestion. Already ...