Electromembrane extraction: Enhanced theoretical understanding and new applications
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- Farmasøytisk institutt [1934]
Abstract
Main research findings In the field of bioanalysis, drug molecules and endogenous compounds can be determined and quantified from biological samples such as blood and urine. However, the complexity of such biological samples makes selective determination of target compounds challenging. Therefore, a sample preparation step is often implemented to extract and separate target compounds from the rest of the sample before analysis. The work in this thesis has been focused on the further development of the sample preparation technique Electromembrane Extraction (EME). In EME, an electrical field is applied across an organic solvent immobilized within a porous membrane, which is used to selectively and rapidly separate target compounds based on their electrical charge and polarity. Even though previous work has demonstrated the high functionality and versatility of EME, more work is deemed necessary in order to move EME from an academic setting over to routine laboratories and analytical industries. This present work has contributed to this pursuit by increasing the theoretical knowledge of EME related to pH conditions and its effects on the selective extraction of non-polar basic analytes. Additionally, the EME concept was tuned towards sample purification of unwanted matrix components, thereby increasing the sample preparation possibilities of EME.List of papers
Paper I. Comprehensive study of buffer systems and local pH effects in electromembrane extraction. Magnus Saed Restan, Henrik Jensen, Xiantao Shen, Chuixiu Huang, Ørjan Grøttem Martinsen, Pavel Kubáň, Astrid Gjelstad, Stig Pedersen-Bjergaard. Analytica Chimica Acta 2017, 984, 116-123. DOI: 10.1016/j.aca.2017.06.049. The article is included in the thesis. Also available at: https://doi.org/10.1016/j.aca.2017.06.049 |
Paper II. Electromembrane extraction of unconjugated fluorescein isothiocyanate from solutions of labeled proteins prior to flow induced dispersion analysis. Magnus Saed Restan, Morten E. Pedersen, Henrik Jensen, Stig Pedersen-Bjergaard. Analytical Chemistry 2019, 91, 6702-6708. DOI: 10.1021/acs.analchem.9b00730. The article is not available in DUO due to publisher restrictions. The published version is available at: https://doi.org/10.1021/acs.analchem.9b00730 |
Paper III. Towards exhaustive electromembrane extraction under stagnant conditions. Magnus Saed Restan, Øystein Skjærvø, Ørjan Grøttem Martinsen, Stig Pedersen-Bjergaard. Analytica Chimica Acta 2020, 1104, 1-9. DOI: 10.1016/j.aca.2020.01.058. The article is included in the thesis. Also available at: https://doi.org/10.1016/j.aca.2020.01.058 |
Paper IV. Electromembrane extraction of sodium dodecyl sulfate from highly concentrated solutions. Magnus Saed Restan, Frøydis Sved Skottvol, Henrik Jensen, Stig Pedersen-Bjergaard. Analyst 2020, 145, 4957-4963. DOI: 10.1039/d0an00622j. The article is included in the thesis. Also available at: https://doi.org/10.1039/d0an00622j |
Paper V. Influence of acid-base dissociation equilibria during electromembrane extraction. Magnus Saed Restan, Sindre Bergstrøm Ramsrud, Henrik Jensen, and Stig Pedersen-Bjergaard. Journal of Separation Science 2020, 43. DOI: 10.1002/jssc.202000391. The article is included in the thesis. Also available at: https://doi.org/10.1002/jssc.202000391 |