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Paper-based smart sampling for bottom-up protein analysis

Skjærvø, Øystein
Doctoral thesis
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PhD-Skjarvo-2020.pdf (4.230Mb)
Year
2020
Permanent link
http://urn.nb.no/URN:NBN:no-77216

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  • Farmasøytisk institutt [1361]
Abstract
Paper-based sample collection has gained increased attention during the past few decades for its simplicity and applicability to a wide range of matrices and analytes. Particularly for sampling of blood. Compared to traditional venous sample collection, paper based sampling introduces many benefits such as; it is non-invasive, it uses small volumes, has increased storage stability and is much less biohazardous. When combined with mass spectrometry for determination of low abundant protein biomarkers, the sample preparation from paper prior to analysis is time consuming and most often demanding. In this work, polymer coated filter paper has been immobilized with proteases and antibodies to serve as the sampling materials and to accelerate the current workflow. The sampling materials was tested and compared to conventional analytical strategies and proved promising performance of determination of low abundant biomarkers in serum and freshly collected whole blood. The sampling materials showed significantly less manual laboratory labour and time consumption as well as increased performance. With future optimization, the sampling concept and its ideas have the potential to readily be incorporated as a viable sampling techniques applied to different strategies such as early stage cancer identification and doping analysis.
List of papers
Paper I. Instant on-paper protein digestion during blood spot sampling. Øystein Skjærvø, Cecilie Rosting, Trine Grønhaug Halvorsen and Léon Reubsaet. Analyst, 142(20) p3837-3847, DOI: 10.1039/c7an01075c. The article is not available in DUO due to publisher restrictions. The published version is available at: https://doi.org/10.1039/c7an01075c
Paper II. Smart blood spots for whole blood protein analysis. Øystein Skjærvø, Trine Grønhaug Halvorsen and Léon Reubsaet. Analyst, 143(13) p3184-3190, DOI: 10.1039/c8an00317c. The article is not available in DUO due to publisher restrictions. The published version is available at: https://doi.org/10.1039/c8an00317c
Paper III. Pre-lab proteolysis for dried serum spots - An intelligent sampler targeting low abundant biomarkers. Øystein Skjærvø, Trine Grønhaug Halvorsen and Léon Reubsaet. Analytical Methods, DOI: 10.1039/c9ay01976f. The article is not available in DUO due to publisher restrictions. The published version is available at: https://doi.org/10.1039/c9ay01976f
Paper IV. Paper-based immunocapture for targeted protein analysis. Øystein Skjærvø, Eirik Solbakk, Trine Grønhaug Halvorsen and Léon Reubsaet. Talanta: The International Journal of Pure and Applied Analytical Chemistry, 195 p764-770, DOI: 10.1016/j.talanta.2018.12.013. The article is included in the thesis. Also available at: https://doi.org/10.1016/j.talanta.2018.12.013
Paper V. All-in-one paper-based sampling chip for targeted protein analysis. Øystein Skjærvø, Trine Grønhaug Halvorsen and Léon Reubsaet, Analytica Chimica Acta, 1089 p56-65, DOI: 10.1016/j.aca.2019.08.043. The article is included in the thesis. Also available at: https://doi.org/10.1016/j.aca.2019.08.043
 
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