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dc.date.accessioned2015-03-02T14:52:34Z
dc.date.available2015-03-02T14:52:34Z
dc.date.created2014-05-08T08:38:40Z
dc.date.issued2014
dc.identifier.citationRiksen, Elisabeth Aurstad Landin, Maria Augusta Dos S Silva Reppe, Sjur Nakamura, Yoikio Lyngstadaas, Ståle Petter Reseland, Janne Elin . Enamel Matrix Derivative Promote Primary Human Pulp Cell Differentiation and Mineralization. International Journal of Molecular Sciences. 2014, 15(5), 7731-7749
dc.identifier.urihttp://hdl.handle.net/10852/42657
dc.description.abstractEnamel matrix derivative (EMD) has been found to induce reactive dentin formation; however the molecular mechanisms involved are unclear. The effect of EMD (5–50 µg/mL) on primary human pulp cells were compared to untreated cells and cells incubated with 10-8 M dexamethasone (DEX) for 1, 2, 3, 7, and 14 days in culture. Expression analysis using Affymetrix microchips demonstrated that 10 µg/mL EMD regulated several hundred genes and stimulated the gene expression of proteins involved in mesenchymal proliferation and differentiation. Both EMD and DEX enhanced the expression of amelogenin (amel), and the dentinogenic markers dentin sialophosphoprotein (DSSP) and dentin matrix acidic phosphoprotein 1 (DMP1), as well as the osteogenic markers osteocalcin (OC, BGLAP) and collagen type 1 (COL1A1). Whereas, only EMD had effect on alkaline phosphatase (ALP) mRNA expression, the stimulatory effect were verified by enhanced secretion of OC and COL1A from EMD treated cells, and increased ALP activity in cell culture medium after EMD treatment. Increased levels of interleukin-6 (IL-6), interleukin-8 (IL-8), and monocyte chemoattractant proteins (MCP-1) in the cell culture medium were also found. Consequently, the suggested effect of EMD is to promote differentiation of pulp cells and increases the potential for pulpal mineralization to favor reactive dentine formation.en_US
dc.languageEN
dc.language.isoenen_US
dc.rightsAttribution 3.0 Unported
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/
dc.titleEnamel Matrix Derivative Promote Primary Human Pulp Cell Differentiation and Mineralizationen_US
dc.typeJournal articleen_US
dc.creator.authorRiksen, Elisabeth Aurstad
dc.creator.authorLandin, Maria Augusta Dos S Silva
dc.creator.authorReppe, Sjur
dc.creator.authorNakamura, Yoikio
dc.creator.authorLyngstadaas, Ståle Petter
dc.creator.authorReseland, Janne Elin
cristin.unitcode185,16,17,62
cristin.unitnameBiomaterialer
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1
dc.identifier.cristin1131744
dc.identifier.bibliographiccitationinfo:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=International Journal of Molecular Sciences&rft.volume=15&rft.spage=7731&rft.date=2014
dc.identifier.jtitleInternational Journal of Molecular Sciences
dc.identifier.volume15
dc.identifier.issue5
dc.identifier.startpage7731
dc.identifier.endpage7749
dc.identifier.doihttp://dx.doi.org/10.3390/ijms15057731
dc.identifier.urnURN:NBN:no-47050
dc.type.documentTidsskriftartikkelen_US
dc.type.peerreviewedPeer reviewed
dc.source.issn1422-0067
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/42657/1/ijms-15-07731.pdf
dc.type.versionPublishedVersion


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