Hide metadata

dc.contributor.authorNordeng, Jostein
dc.contributor.authorSolheim, Svein
dc.contributor.authorÅkra, Sissel
dc.contributor.authorSchandiz, Hossein
dc.contributor.authorHoffmann, Pavel
dc.contributor.authorRoald, Borghild
dc.contributor.authorBendz, Bjørn
dc.contributor.authorArnesen, Harald
dc.contributor.authorHelseth, Ragnhild
dc.contributor.authorSeljeflot, Ingebjørg
dc.date.accessioned2022-05-03T05:03:17Z
dc.date.available2022-05-03T05:03:17Z
dc.date.issued2022
dc.identifier.citationThrombosis Journal. 2022 Apr 29;20(1):23
dc.identifier.urihttp://hdl.handle.net/10852/93856
dc.description.abstractBackground The fibrinolytic system plays an important role in coronary artery atherothrombosis, and especially circulating plasminogen-activator inhibitor (PAI) type 1 (PAI-1) associates with increased mortality, infarct size and heart failure in patients with myocardial infarction (MI). In a cross-sectional study, we aimed to study whether genes encoding tissue plasminogen activator (tPA), urinary-type plasminogen activator (uPA), PAI-1 and PAI-2 are expressed in coronary thrombi from acute ST-elevation MI (STEMI) patients. Any relations to myocardial injury measured by peak troponin T, time from symptom onset to Percutaneous Coronary Intervention (PCI), and to different cell types present in the thrombi were also explored. Methods Intracoronary thrombi were aspirated from 33 STEMI patients treated with primary PCI. The thrombi were snap-frozen for gene expression analyses, relatively quantified by RT PCR. Peripheral blood samples were drawn. Correlations were performed by Spearmans rho. Results The genes were present in 74–94% of the thrombi. Median peak troponin T was 3434 μ/L and median ischemic time 152 min. There were no significant correlations between the measured genes and troponin T, or ischemic time. Genes encoding tPA, u-PA, PAI-1 and PAI-2 all correlated significantly to the presence of monocytes/macrophages (CD68) in the thrombi (p = 0.028, p < 0.001, p = 0.003, p < 0.001). PAI-1 and PAI-2 also correlated to endothelial cells (CD31) (p = 0.002, p = 0.016). uPA associated with neutrophil granulocytes (CD 66b) (p = 0.019). Conclusion Genes encoding tPA, uPA, PAI-1 and PAI-2 were highly expressed in human coronary thrombi from STEMI patients, indicating fibrinolytic regulators playing active roles in the thrombi, although not related to myocardial injury. All markers related to the presence of monocytes/macrophages, indicating connection to local inflammatory cells. Trial registration The study is registered at clinicaltrials.gov with identification number NCT02746822.
dc.language.isoeng
dc.rightsThe Author(s); licensee BioMed Central Ltd.
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleGene expression of fibrinolytic markers in coronary thrombi
dc.typeJournal article
dc.date.updated2022-05-03T05:03:18Z
dc.creator.authorNordeng, Jostein
dc.creator.authorSolheim, Svein
dc.creator.authorÅkra, Sissel
dc.creator.authorSchandiz, Hossein
dc.creator.authorHoffmann, Pavel
dc.creator.authorRoald, Borghild
dc.creator.authorBendz, Bjørn
dc.creator.authorArnesen, Harald
dc.creator.authorHelseth, Ragnhild
dc.creator.authorSeljeflot, Ingebjørg
dc.identifier.cristin2024587
dc.identifier.doihttps://doi.org/10.1186/s12959-022-00383-1
dc.identifier.urnURN:NBN:no-96413
dc.type.documentTidsskriftartikkel
dc.type.peerreviewedPeer reviewed
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/93856/1/12959_2022_Article_383.pdf
dc.type.versionPublishedVersion
cristin.articleid23


Files in this item

Appears in the following Collection

Hide metadata

Attribution 4.0 International
This item's license is: Attribution 4.0 International