Hide metadata

dc.date.accessioned2022-02-03T16:39:46Z
dc.date.available2022-02-03T16:39:46Z
dc.date.created2022-01-14T09:55:31Z
dc.date.issued2022
dc.identifier.citationNielsen, Josefine Eilsø Lund, Reidar . Molecular Transport and Growth of Lipid Vesicles Exposed to Antimicrobial Peptides. Langmuir. 2022, 38(1), 374-384
dc.identifier.urihttp://hdl.handle.net/10852/90487
dc.description.abstractIt is well-known that lipids constituting the cytoplasmic membrane undergo continuous reorganization to maintain the appropriate composition important for the integrity of the cell. The transport of lipids is controlled by mainly membrane proteins, but also spontaneous lipid transport between leaflets, lipid “flip–flop”, occurs. These processes do not only occur spontaneously under equilibrium, but also promote structural rearrangements, morphological transitions, and growth processes. It has previously been shown that intravesicular lipid “flip–flop” and intervesicular lipid exchange under equilibrium can be deduced indirectly from contrast variation time-resolved small-angle neutron scattering (TR-SANS) where the molecules are “tagged” using hydrogen/deuterium (H/D) substitution. In this work, we show that this technique can be extended to simultaneously detect changes in the growth and the lipid “flip–flop” and exchange rates induced by a peptide additive on lipid vesicles consisting of DMPC (1,2-dimyristoyl-sn-glycero-3-phosphocholine), d-DMPC (1,2-dimyristoyl-d54-sn-glycero-3-phosphocholine), DMPG (1,2-dimyristoyl-sn-glycero-3-phospho-(1′-rac-glycerol)), and small amounts of DMPE-PEG (1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000]). Changes in the overall size were independently monitored using dynamic light scattering (DLS). We find that the antimicrobial peptide, indolicidin, accelerates lipid transport and additionally induces limited vesicular growth. Moreover, in TR-SANS experiments using partially labeled lipid mixtures to separately study the kinetics of the lipid components, we show that, whereas peptide addition affects both lipids similarly, DMPG exhibits faster kinetics. We find that vesicular growth is mainly associated with peptide-mediated lipid reorganization that only slightly affects the overall exchange kinetics. This is confirmed by a TR-SANS experiment of vesicles preincubated with peptide showing that after pre-equilibration the kinetics are only slightly slower.
dc.languageEN
dc.publisherACS Publications
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleMolecular Transport and Growth of Lipid Vesicles Exposed to Antimicrobial Peptides
dc.typeJournal article
dc.creator.authorNielsen, Josefine Eilsø
dc.creator.authorLund, Reidar
cristin.unitcode185,15,12,63
cristin.unitnameSeksjon for kjemisk livsvitenskap - biomolekyler, bio-inspirerte materialer og bioanalytisk kjemi
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode2
dc.identifier.cristin1980960
dc.identifier.bibliographiccitationinfo:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Langmuir&rft.volume=38&rft.spage=374&rft.date=2022
dc.identifier.jtitleLangmuir
dc.identifier.volume38
dc.identifier.issue1
dc.identifier.startpage374
dc.identifier.endpage384
dc.identifier.doihttps://doi.org/10.1021/acs.langmuir.1c02736
dc.identifier.urnURN:NBN:no-93097
dc.type.documentTidsskriftartikkel
dc.type.peerreviewedPeer reviewed
dc.source.issn0743-7463
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/90487/1/acs.langmuir.1c02736.pdf
dc.type.versionPublishedVersion


Files in this item

Appears in the following Collection

Hide metadata

Attribution 4.0 International
This item's license is: Attribution 4.0 International