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dc.date.accessioned2022-01-24T18:53:19Z
dc.date.available2022-01-24T18:53:19Z
dc.date.created2021-09-02T15:30:54Z
dc.date.issued2022
dc.identifier.citationDanbolt, Niels Christian López-Corcuera, Beatriz Zhou, Yun . Reconstitution of GABA, Glycine and Glutamate Transporters. Neurochemical Research. 2021
dc.identifier.urihttp://hdl.handle.net/10852/90011
dc.description.abstractAbstract In contrast to water soluble enzymes which can be purified and studied while in solution, studies of solute carrier (transporter) proteins require both that the protein of interest is situated in a phospholipid membrane and that this membrane forms a closed compartment. An additional challenge to the study of transporter proteins has been that the transport depends on the transmembrane electrochemical gradients. Baruch I. Kanner understood this early on and first developed techniques for studying plasma membrane vesicles. This advanced the field in that the experimenter could control the electrochemical gradients. Kanner, however, did not stop there, but started to solubilize the membranes so that the transporter proteins were taken out of their natural environment. In order to study them, Kanner then had to find a way to reconstitute them (reinsert them into phospholipid membranes). The scope of the present review is both to describe the reconstitution method in full detail as that has never been done, and also to reveal the scientific impact that this method has had. Kanner’s later work is not reviewed here although that also deserves a review because it too has had a huge impact.
dc.languageEN
dc.publisherKluwer Academic/Plenum Publishers
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleReconstitution of GABA, Glycine and Glutamate Transporters
dc.typeJournal article
dc.creator.authorDanbolt, Niels Christian
dc.creator.authorLópez-Corcuera, Beatriz
dc.creator.authorZhou, Yun
cristin.unitcode185,51,12,30
cristin.unitnameSeksjon for anatomi
cristin.ispublishedfalse
cristin.fulltextoriginal
cristin.qualitycode1
dc.identifier.cristin1930915
dc.identifier.bibliographiccitationinfo:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Neurochemical Research&rft.volume=&rft.spage=&rft.date=2021
dc.identifier.jtitleNeurochemical Research
dc.identifier.volume47
dc.identifier.issue1
dc.identifier.startpage85
dc.identifier.endpage110
dc.identifier.doihttps://doi.org/10.1007/s11064-021-03331-z
dc.identifier.urnURN:NBN:no-92615
dc.type.documentTidsskriftartikkel
dc.type.peerreviewedPeer reviewed
dc.source.issn0364-3190
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/90011/1/Danbolt2021_Article_ReconstitutionOfGABAGlycineAnd.pdf
dc.type.versionPublishedVersion
dc.relation.projectUIO/Unger Vetlesen Medical Fund
dc.relation.projectUIO/SERTA; UNIFOR-FRIMED, Nansenfondet and Anders Jahres Fond
dc.relation.projectNFR/240844
dc.relation.projectEU/SAF2017-84235-R


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