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dc.date.accessioned2021-12-22T16:11:13Z
dc.date.available2021-12-22T16:11:13Z
dc.date.created2021-06-09T11:36:41Z
dc.date.issued2021
dc.identifier.citationAlswady-Hoff, Mayes Erdem, Johanna Maria Samulin Phuyal, Santosh Knittelfelder, Oskar Sharma, Animesh Fonseca, Davi de Miranda Skare, Øivind Slupphaug, Geir Zienolddiny, Shanbeh . Long-Term Exposure to Nanosized TiO2 Triggers Stress Responses and Cell Death Pathways in Pulmonary Epithelial Cells. International Journal of Molecular Sciences. 2021, 22(10)
dc.identifier.urihttp://hdl.handle.net/10852/89781
dc.description.abstractThere is little in vitro data available on long-term effects of TiO2 exposure. Such data are important for improving the understanding of underlying mechanisms of adverse health effects of TiO2 . Here, we exposed pulmonary epithelial cells to two doses (0.96 and 1.92 µg/cm2 ) of TiO2 for 13 weeks and effects on cell cycle and cell death mechanisms, i.e., apoptosis and autophagy were determined after 4, 8 and 13 weeks of exposure. Changes in telomere length, cellular protein levels and lipid classes were also analyzed at 13 weeks of exposure. We observed that the TiO2 exposure increased the fraction of cells in G1-phase and reduced the fraction of cells in G2-phase, which was accompanied by an increase in the fraction of late apoptotic/necrotic cells. This corresponded with an induced expression of key apoptotic proteins i.e., BAD and BAX, and an accumulation of several lipid classes involved in cellular stress and apoptosis. These findings were further supported by quantitative proteome profiling data showing an increase in proteins involved in cell stress and genomic maintenance pathways following TiO2 exposure. Altogether, we suggest that cell stress response and cell death pathways may be important molecular events in long-term health effects of TiO2
dc.languageEN
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleLong-Term Exposure to Nanosized TiO2 Triggers Stress Responses and Cell Death Pathways in Pulmonary Epithelial Cells
dc.typeJournal article
dc.creator.authorAlswady-Hoff, Mayes
dc.creator.authorErdem, Johanna Maria Samulin
dc.creator.authorPhuyal, Santosh
dc.creator.authorKnittelfelder, Oskar
dc.creator.authorSharma, Animesh
dc.creator.authorFonseca, Davi de Miranda
dc.creator.authorSkare, Øivind
dc.creator.authorSlupphaug, Geir
dc.creator.authorZienolddiny, Shanbeh
cristin.unitcode185,51,12,0
cristin.unitnameAvdeling for molekylærmedisin
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1
dc.identifier.cristin1914765
dc.identifier.bibliographiccitationinfo:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=International Journal of Molecular Sciences&rft.volume=22&rft.spage=&rft.date=2021
dc.identifier.jtitleInternational Journal of Molecular Sciences
dc.identifier.volume22
dc.identifier.issue10
dc.identifier.pagecount16
dc.identifier.doihttps://doi.org/10.3390/ijms22105349
dc.identifier.urnURN:NBN:no-92386
dc.type.documentTidsskriftartikkel
dc.type.peerreviewedPeer reviewed
dc.source.issn1422-0067
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/89781/2/ijms-22-05349-v2.pdf
dc.type.versionPublishedVersion
cristin.articleid5349
dc.relation.projectNOTUR/NORSTORE/NN9036K


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