Background: Antimicrobial resistance (AMR) is one of the leading causes of death worldwide. The AMR dilemma has become a major concern in the low-to-middle income countries (LMICs). The WHO is promoting effective global actions against infectious diseases with the appropriate use of antimicrobials. Community pharmacists can play a unique role in restricting the use of fluoroquinolones through responsible counselling and dispensing. This study has investigated the pharmacists’ knowledge and attitudes toward FQs’ resistance, as there is a huge research gap since a few studies have been conducted on pharmacists’ knowledge and attitudes toward AMR in Pakistan. Method: A qualitative study design was employed by conducting in-depth interviews to explore the knowledge and attitudes of community pharmacists toward FQs’ resistance. Findings: Twenty in-depth-interviews were conducted, with 17 interviews taken from retail pharmacists, two from pharmacy technicians and one from a wholesaler. The study participants demonstrated a wide range of knowledge and attitudes regarding FQs and AMR. According to them, a broad spectrum of antibiotics is the most prescribed antimicrobials. Self-medication, excessive prescribing, irresponsible dispensing and the over-the-counter (OTC) availability of antimicrobials with the lack of regulations were the key factors contributing to the enhancement of FQs’ resistance. The pharmacists wanted to be part of a system that addressed this problem in this alarming situation. Conclusion: The community pharmacists had good knowledge about AMR, although there were gaps in the dispensing practices of community pharmacists. In this current scenario, it is necessary to take serious steps to fill the gaps and tackle the problems of excessive prescribing, as well as the use of antimicrobials, by introducing effective policies. The results of the study can play an important role in informing local policies directed at AMR, and ultimately in motivating pharmacists to play a proactive role in AMR reduction.