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dc.date.accessioned2021-01-19T20:44:34Z
dc.date.available2021-01-19T20:44:34Z
dc.date.created2021-01-05T11:16:34Z
dc.date.issued2020
dc.identifier.citationYadav, Ajay Amber, Mazia Zosen, Denis Labba, Nils Anders Huiberts, Eva Henriette Willemijn Samulin-Erdem, Johanna Maria Haugen, Fred Berntsen, Hanne Friis Zienolddiny, Shanbeh Paulsen, Ragnhild Elisabeth Ropstad, Erik Connolly, Lisa Verhaegen, Steven . A human relevant mixture of persistent organic pollutants (POPs) and perfluorooctane sulfonic acid (PFOS) enhance nerve growth factor (NGF)-induced neurite outgrowth in PC12 cells. Toxicology Letters. 2020, 338, 85-96
dc.identifier.urihttp://hdl.handle.net/10852/82358
dc.description.abstractDisruption of neurite outgrowth is a marker for neurotoxicity. Persistent organic pollutants (POPs) are potential developmental neurotoxicants. We investigated their effect on neurite outgrowth in PC12 rat pheochromocytoma cells, in absence or presence of nerve growth factor (NGF), an inducer of neuronal differentiation. Cells were exposed for 72 h to a defined mixture of POPs with chemical composition and concentrations based on blood levels in the Scandinavian population. We also evaluated perfluorooctane sulfonic acid (PFOS) alone, the most abundant compound in the POP mixture. Only higher concentrations of POP mixture reduced tetrazolium salt (MTT) conversion. High-content analysis showed a decrease in cell number, but no changes for nuclear and mitochondrial cellular health parameters. Robust glutathione levels were observed in NGF-differentiated cells. Live imaging, using the IncuCyte ZOOM platform indicated ongoing cell proliferation over time, but slower in presence of NGF. The pollutants did not inhibit neuritogenesis, but rather increased NGF-induced neurite length. PFOS induced neurite outgrowth to about 50 % of the level seen with the POP mixture. Neither the POP mixture nor PFOS affected neurite length in the absence of NGF. Our observations indicate that realistic complex mixtures of environmental pollutants can affect neuronal connectivity via NGF-induced neurite outgrowth.
dc.languageEN
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleA human relevant mixture of persistent organic pollutants (POPs) and perfluorooctane sulfonic acid (PFOS) enhance nerve growth factor (NGF)-induced neurite outgrowth in PC12 cells
dc.typeJournal article
dc.creator.authorYadav, Ajay
dc.creator.authorAmber, Mazia
dc.creator.authorZosen, Denis
dc.creator.authorLabba, Nils Anders
dc.creator.authorHuiberts, Eva Henriette Willemijn
dc.creator.authorSamulin-Erdem, Johanna Maria
dc.creator.authorHaugen, Fred
dc.creator.authorBerntsen, Hanne Friis
dc.creator.authorZienolddiny, Shanbeh
dc.creator.authorPaulsen, Ragnhild Elisabeth
dc.creator.authorRopstad, Erik
dc.creator.authorConnolly, Lisa
dc.creator.authorVerhaegen, Steven
cristin.unitcode185,15,23,30
cristin.unitnameFarmakologi og farmasøytisk biovitenskap
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1
dc.identifier.cristin1865385
dc.identifier.bibliographiccitationinfo:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Toxicology Letters&rft.volume=338&rft.spage=85&rft.date=2020
dc.identifier.jtitleToxicology Letters
dc.identifier.volume338
dc.identifier.startpage85
dc.identifier.endpage96
dc.identifier.doihttps://doi.org/10.1016/j.toxlet.2020.12.007
dc.identifier.urnURN:NBN:no-85242
dc.type.documentTidsskriftartikkel
dc.type.peerreviewedPeer reviewed
dc.source.issn0378-4274
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/82358/2/1-s2.0-S0378427420305002-main.pdf
dc.type.versionPublishedVersion
dc.relation.projectEC/H2020/722634


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