Membrane budding dynamics by diffusion and kinetic recruitment of proteins
Appears in the following Collection
- Matematisk institutt 
AbstractBiological membranes have fundamental implications in a large number of processes in the cell. As such, membranes possess important properties: they can change their shape as an elastic surface, and they are fluid, which allows the diffusion of different molecules and proteins. In this work, I study, from a theoretical point of view, some of the implications of the membrane fluidity and elasticity. In particular, I focus on the influence of characteristic fixed membrane shapes, similar to the ones observed in some experiments, on the diffusion of molecules. This study reveals a dependence between the diffusion time of the molecules and the membrane curvature. Membrane deformations, on the other hand, can be produced by certain type of proteins, which can be recruited into the membrane. As consequence, I also focus on the effect of diffusion and recruitment of proteins in the membrane shape evolution. This study reveals gradual shape deformation leading to the formation of spherical vesicles. It also suggests a dependence between the rate of protein recruitment and the time required to form the vesicle.
List of papers
|Paper I Rojas, R., Liese, S. and Carlson, A. “Diffusion on membrane domes, tubes and pearling structures”. In: bioRxiv, currently in review. (2020). To be published. The paper is removed from the thesis in DUO awaiting publishing.|
|Paper II Rojas, R., Liese, S., Alimohamadi, H., Rangamani, P. and Carlson, A. “Diffuso-kinetic membrane budding dynamics”. In: Soft Matter (2020) DOI: 10.1039/D0SM01028F The paper is included in the thesis in DUO, and also available at: https://doi.org/10.1039/D0SM01028F|
|Paper III Liese, S., Wenzel, E. M., Kjos, I., Rojas R., Schultz, S., Brech, A., Stenmark, H., Raiborg, C. and Carlson, A. “Protein crowding mediates membrane remodeling in upstream ESCRT-induced formation of intraluminal vesicles”. In: Proceedings of the National Academy of Sciences of the United States of America (2020) DOI: 10.1073/pnas.2014228117 The paper is removed from the thesis in DUO due to publisher restrictions. The published version is available at: https://doi.org/10.1073/pnas.2014228117|