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dc.date.accessioned2020-10-16T18:14:16Z
dc.date.available2020-10-16T18:14:16Z
dc.date.created2020-10-13T12:32:04Z
dc.date.issued2020
dc.identifier.citationQuiles Jimenez, Ana Maria Teresa Gregersen, Ida Sousa, Mirta Abbas, Azhar Kong, Xiang Yi Alseth, Ingrun Holm, Sverre Dahl, Tuva Børresdatter Skagen, Karolina Ryeng Skjelland, Mona Aukrust, Pål Bjørås, Magnar Halvorsen, Bente . N6-methyladenosine in RNA of atherosclerotic plaques: An epitranscriptomic signature of human carotid atherosclerosis. Biochemical and Biophysical Research Communications - BBRC. 2020, 1-7
dc.identifier.urihttp://hdl.handle.net/10852/80637
dc.description.abstractBackground More than 170 post-transcriptional RNA modifications regulate the localization, processing and function of cellular RNAs, and aberrant RNA modifications have been linked to a range of human diseases. The RNA modification landscape in atherosclerosis, the main underlying cause of cardiovascular diseases, is still largely unknown. Methods We used mass spectrometry to analyse a selection of RNA-modifying enzymes and the N6-methyladenosine (m6A) in carotid atherosclerotic lesion samples representing early and advanced stages of atherosclerosis as compared to non-atherosclerotic arteries from healthy controls. Findings (i) the detection of different levels of several enzymes involved in methylations occurring in rRNA and mRNA; (ii) these findings included changes in the levels of methyltransferases (‘writers’), binding proteins (‘readers’) and demethylases (‘erasers’) during atherosclerosis as compared to non-atherosclerotic control arteries, with generally the most prominent differences in samples from early atherosclerotic lesions; and (iii) these changes were accompanied by a marked downregulation of m6A in rRNA, the most abundant and well-studied modification in mRNA with a wide range of effects on cell biology. Interpretation We show for the first time that RNA-modifying enzymes and the well-studied RNA modification m6A are differentially regulated in atherosclerotic lesions, which potentially could help creating new prognostic and treatment strategies.
dc.languageEN
dc.relation.ispartofQuiles-Jiménez, Ana María (2020) Molecular mechanisms of atherosclerotic disease: Studies on the role of the DNA glycosylase NEIL3 and the epitranscriptome in the development of atherosclerosis. Doctoral thesis http://hdl.handle.net/10852/81169
dc.relation.urihttp://hdl.handle.net/10852/81169
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.titleN6-methyladenosine in RNA of atherosclerotic plaques: An epitranscriptomic signature of human carotid atherosclerosis
dc.typeJournal article
dc.creator.authorQuiles Jimenez, Ana Maria Teresa
dc.creator.authorGregersen, Ida
dc.creator.authorSousa, Mirta
dc.creator.authorAbbas, Azhar
dc.creator.authorKong, Xiang Yi
dc.creator.authorAlseth, Ingrun
dc.creator.authorHolm, Sverre
dc.creator.authorDahl, Tuva Børresdatter
dc.creator.authorSkagen, Karolina Ryeng
dc.creator.authorSkjelland, Mona
dc.creator.authorAukrust, Pål
dc.creator.authorBjørås, Magnar
dc.creator.authorHalvorsen, Bente
cristin.unitcode185,53,48,14
cristin.unitnameInstitutt for indremedisinsk forskning
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1
dc.identifier.cristin1839170
dc.identifier.bibliographiccitationinfo:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Biochemical and Biophysical Research Communications - BBRC&rft.volume=&rft.spage=1&rft.date=2020
dc.identifier.jtitleBiochemical and Biophysical Research Communications - BBRC
dc.identifier.startpage1
dc.identifier.endpage7
dc.identifier.doihttps://doi.org/10.1016/j.bbrc.2020.09.057
dc.identifier.urnURN:NBN:no-83728
dc.type.documentTidsskriftartikkel
dc.type.peerreviewedPeer reviewed
dc.source.issn0006-291X
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/80637/1/1-s2.0-S0006291X20317988-main.pdf
dc.type.versionPublishedVersion


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