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Nano liquid chromatography columns

Wilson, Steven Ray Haakon; Olsen, Christine; Lundanes, Elsa
Journal article; AcceptedVersion; Peer reviewed
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NanoLC_column_review_postprint_version.pdf (1.532Mb)
Year
2019
Permanent link
http://urn.nb.no/URN:NBN:no-80697

CRIStin
1760169

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Appears in the following Collection
  • Kjemisk institutt [825]
  • Institutt for medisinske basalfag [1338]
  • CRIStin høstingsarkiv [14929]
Original version
The Analyst. 2019, 144 (24), 7090-7104, DOI: https://doi.org/10.1039/c9an01473j
Abstract
Nano liquid chromatography (nanoLC), with columns having an inner diameter (ID) of ≤100 μm, can provide enhanced sensitivity and enable analysis of limited samples. NanoLC has become an established tool in omics research, and is gaining ground in other applications as well. There are several variants and formats of nanoLC columns, including packed columns, monoliths, open tubular columns, and the pillar array format. Most applications are done with packed columns, while e.g. the monolith and open tubular columns are still less established as routine tools. The pillar array format is a new variant with excellent resolution and low backpressure, and has recently been commercialized and used for bio-applications. In this minireview, we summarize and discuss recent research on nanoLC column development and uses, focusing on literature between 2016 and medio 2019.
 
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