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dc.date.accessioned2020-05-25T19:54:04Z
dc.date.available2020-05-25T19:54:04Z
dc.date.created2019-10-15T16:56:54Z
dc.date.issued2019
dc.identifier.citationKöksal, Elif Belletati, Patrícia Fernandes Reint, Ganna Olsson, Ragni Leitl, Kira Kantarci, Ilayda Gözen, Irep . Spontaneous Formation and Rearrangement of Artificial Lipid Nanotube Networks as a Bottom-Up Model for Endoplasmic Reticulum. Journal of Visualized Experiments. 2019, 2019(143), 1-9
dc.identifier.urihttp://hdl.handle.net/10852/76267
dc.description.abstractWe present a convenient method to form a bottom-up structural organelle model for the endoplasmic reticulum (ER). The model consists of highly dense lipidic nanotubes that are, in terms of morphology and dynamics, reminiscent of ER. The networks are derived from phospholipid double bilayer membrane patches adhering to a transparent Al2O3 substrate. The adhesion is mediated by Ca2+ in the ambient buffer. Subsequent depletion of Ca2+ by means of BAPTA/EDTA causes retraction of the membrane, resulting in spontaneous lipid nanotube network formation. The method only comprises phospholipids and microfabricated surfaces for simple formation of an ER model and does not require the addition of proteins or chemical energy (e.g., GTP or ATP). In contrast to the 3D morphology of the cellular endoplasmic reticulum, the model is two-dimensional (albeit the nanotube dimensions, geometry, structure, and dynamics are maintained). This unique in vitro ER model consists of only a few components, is easy to construct, and can be observed under a light microscope. The resulting structure can be further decorated for additional functionality, such as the addition of ER-associated proteins or particles to study transport phenomena among the tubes. The artificial networks described here are suitable structural models for the cellular ER, whose unique characteristic morphology has been shown to be related to its biological function, whereas details regarding formation of the tubular domain and rearrangements within are still not completely understood. We note that this method uses Al2O3 thin-film-coated microscopy coverslips, which are commercially available but require special orders. Therefore, it is advisable to have access to a microfabrication facility for preparation.
dc.languageEN
dc.publisherJournal of Visualized Experiments
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Unported
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/3.0/
dc.titleSpontaneous Formation and Rearrangement of Artificial Lipid Nanotube Networks as a Bottom-Up Model for Endoplasmic Reticulum
dc.typeJournal article
dc.creator.authorKöksal, Elif
dc.creator.authorBelletati, Patrícia Fernandes
dc.creator.authorReint, Ganna
dc.creator.authorOlsson, Ragni
dc.creator.authorLeitl, Kira
dc.creator.authorKantarci, Ilayda
dc.creator.authorGözen, Irep
cristin.unitcode185,57,0,0
cristin.unitnameNorsk Senter for Molekylærmedisin
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1
dc.identifier.cristin1737322
dc.identifier.bibliographiccitationinfo:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal of Visualized Experiments&rft.volume=2019&rft.spage=1&rft.date=2019
dc.identifier.jtitleJournal of Visualized Experiments
dc.identifier.volume2019
dc.identifier.issue143
dc.identifier.startpage1
dc.identifier.endpage9
dc.identifier.doihttps://doi.org/10.3791/58923
dc.identifier.urnURN:NBN:no-79341
dc.type.documentTidsskriftartikkel
dc.type.peerreviewedPeer reviewed
dc.source.issn1940-087X
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/76267/2/jove-protocol-58923-spontaneous-formation-rearrangement-artificial-lipid-nanotube.pdf
dc.type.versionPublishedVersion
dc.relation.projectNFR/187615
dc.relation.projectNFR/274433


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