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dc.contributor.authorSoreide, Endre
dc.contributor.authorDenbeigh, Janet M
dc.contributor.authorLewallen, Eric A
dc.contributor.authorThaler, Roman
dc.contributor.authorSamsonraj, Rebekah M
dc.contributor.authorJones, Dakota L
dc.contributor.authorXu, Wei
dc.contributor.authorLarson, Dirk
dc.contributor.authorNordsletten, Lars
dc.contributor.authorKakar, Sanjeev
dc.contributor.authorvan Wijnen, Andre J
dc.date.accessioned2019-11-19T06:02:13Z
dc.date.available2019-11-19T06:02:13Z
dc.date.issued2019
dc.identifier.citationBMC Musculoskeletal Disorders. 2019 Nov 18;20(1):549
dc.identifier.urihttp://hdl.handle.net/10852/70882
dc.description.abstractBackground Injuries in the musculoskeletal system, such as tendon and ligament ruptures, are challenging to manage and often require surgical reconstructions with limited long-term success. Thus, characterizations of these tissues are urgently needed to better understand cellular mechanisms that regulate tissue homeostasis and healing. Explant culturing systems allow for ex vivo analysis of tissues in an environment that mimics the native microenvironment in vivo. Methods Collaborative efforts within our institution facilitated the establishment of a novel explant culturing system. Tissue specimens cultured in single wells, with individual applied loading and/or biological environment, allowed characterization of tissue cultured under a variety of biological loading conditions. Quantitative PCR analysis for selected gene markers was our primary outcome. Results Data were stratified for analysis by either culture environment or loading condition. Our gene expression results show that specimens clustered by culture condition may differ in molecular markers related to ECM production (e.g., Col1a1, Adamts4) and/or organization (e.g., Tnc, Dnc). In contrast, loading condition did significantly alter the median gene expression levels of tissues in comparison to unloaded control samples, although gene expression values related to ECM degradation (e.g., Mmp1, Mmp10) were altered in tendons cultured under tension in the device. Conclusion Our study demonstrates promising utility of a novel explant culturing system for further characterization of musculoskeletal tissues such as native tendons and ligaments, as well as pathologic fibrotic tissues resulting from arthrofibrosis or Dupuytren’s disease.
dc.language.isoeng
dc.rightsThe Author(s).
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleA multi-chamber tissue culture device for load-dependent parallel evaluation of tendon explants
dc.typeJournal article
dc.date.updated2019-11-19T06:02:17Z
dc.creator.authorSoreide, Endre
dc.creator.authorDenbeigh, Janet M
dc.creator.authorLewallen, Eric A
dc.creator.authorThaler, Roman
dc.creator.authorSamsonraj, Rebekah M
dc.creator.authorJones, Dakota L
dc.creator.authorXu, Wei
dc.creator.authorLarson, Dirk
dc.creator.authorNordsletten, Lars
dc.creator.authorKakar, Sanjeev
dc.creator.authorvan Wijnen, Andre J
dc.identifier.doihttps://doi.org/10.1186/s12891-019-2896-2
dc.identifier.urnURN:NBN:no-74001
dc.type.documentTidsskriftartikkel
dc.type.peerreviewedPeer reviewed
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/70882/1/12891_2019_Article_2896.pdf
dc.type.versionPublishedVersion
cristin.articleid549


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