Hide metadata

dc.date.accessioned2019-03-25T10:59:55Z
dc.date.created2019-02-01T05:49:00Z
dc.date.issued2019
dc.identifier.citationReubsaet, Leon Sweredoski, Michael J. Moradian, Annie . Data-Independent Acquisition for the Orbitrap Q Exactive HF: A Tutorial.. Journal of Proteome Research. 2019
dc.identifier.urihttp://hdl.handle.net/10852/67375
dc.description.abstractData-independent acquisition (DIA) is a powerful mass spectrometric technique to perform both protein identification and quantification of complex protein samples. Setting up DIA methods on Orbitrap analyzers requires a thorough overview of the actions the Orbitrap mass spectrometers carry out. This Tutorial is written with the intention to give an overview of the important parameters to consider as well as which measurements to carry out to get the most out of your DIA method when setting it up on an Orbitrap mass analyzer. Instead of giving the optimal DIA settings, all steps in the construction and optimization of the DIA method are shown and discussed in a way that allows tailored DIA methods. They key steps are building the spectral library after sample fractionation, deciding upon the number of data points per chromatographic peak, determining the scan times of each mass spectrometric step, constructing various DIA methods using these data, and evaluating their performance. This proposed DIA method development strategy was tested on digested lysates from Pseudomonas aeruginosa and compared with conventional DDA analysis to put the DIA results into perspective.en_US
dc.languageEN
dc.publisherACS Publications
dc.titleData-Independent Acquisition for the Orbitrap Q Exactive HF: A Tutorial.en_US
dc.typeJournal articleen_US
dc.creator.authorReubsaet, Leon
dc.creator.authorSweredoski, Michael J.
dc.creator.authorMoradian, Annie
dc.date.embargoenddate2019-12-17
cristin.unitcode185,15,23,20
cristin.unitnameFarmasøytisk kjemi
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.fulltextpostprint
cristin.qualitycode2
dc.identifier.cristin1671813
dc.identifier.bibliographiccitationinfo:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal of Proteome Research&rft.volume=&rft.spage=&rft.date=2019
dc.identifier.jtitleJournal of Proteome Research
dc.identifier.doihttp://dx.doi.org/ 10.1021/acs.jproteome.8b00845
dc.identifier.urnURN:NBN:no-70553
dc.type.documentTidsskriftartikkelen_US
dc.type.peerreviewedPeer reviewed
dc.source.issn1535-3893
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/67375/1/Data-Independent%2BAcquisition%2Bfor%2Bthe%2BOrbitrap%2BQ%2BExactive%2BHF-%2BA%2BTutorial.pdf
dc.type.versionAcceptedVersion


Files in this item

Appears in the following Collection

Hide metadata