Omega-3 treatment in hypoxicischemic brain injury: An experimental study in newborn piglets
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AbstractNeonatal encephalopathy, and hereunder hypoxia-ischemia, is a major cause of newborn and pediatric morbidity and mortality. Survivor phenotypes range from mild learning disability to severe neurodevelopmental delay. Therapeutic hypothermia is the only established treatment for hypoxic-ischemic encephalopathy, and it significantly reduces both mortality and morbidity in the moderately affected encephalopathic infants. But still large numbers of infants develop sequelae and adjuvant neuroprotective strategies are warranted. In this thesis, Marianne U. Huun and colleagues have used a well-established newborn piglet model of hypoxia-ischemia to explore a novel neuroprotectant, the omega-3 fatty acid docosahexaenoic acid (DHA). In the model DHA was administered in combination with and without therapeutic hypothermia. Lipid peroxides as markers of oxidative stress were measured in urine, blood and brain tissue. H+MRS was applied on frozen brain tissue and neuropathology was analyzed to exploratory assess reductions in oxidative stress and neuroprotection.
List of papers
|Paper I: Marianne Ullestad Huun, Håvard T. Garberg, Javier Escobar, Consuelo Chafer, Maximo Vento, Ingar M. Holme, Ola Didrik Saugstad and Rønnaug Solberg. DHA reduces oxidative stress following hypoxia-ischemia in newborn piglets: a study of lipid peroxidation products in urine and plasma. Journal of Perinatal Medicine, 2018; 46(2): 209–217. DOI: 10.1515/jpm-2016-0334. The article is included in the thesis. Also available at https://doi.org/10.1515/jpm-2016-0334|
|Paper II: Marianne U. Huun, Havard T. Garberg, Giuseppe Buonocore, Mariangela Longini, Elisa Belvisi, Francesco Bazzini, Fabrizio Proietti, Ola Didrik Saugstad and Rønnaug Solberg. Regional differences of hypothermia on oxidative stress following hypoxia-ischemia: a study of DHA and hypothermia on brain lipid peroxidation in newborn piglets. Journal of Perinatal medicine, 2018 Aug 15. DOI: 10.1515/jpm-2017-0355. The article is included in the thesis. Also available at https://doi.org/10.1515/jpm-2017-0355|
|Paper III: Marianne U. Huun, Håvard T. Garberg, Else M. Løberg, Javier Escobar, Jose Martinez-Orgado, Ola D. Saugstad and Rønnaug Solberg. DHA and therapeutic hypothermia in a short-term follow-up piglet model of hypoxia-ischemia: Effects on H+MRS biomarkers. PLoS One, 2018 Aug 7; 13(8): e0201895. The article is included in the thesis. Also available in DUO http://urn.nb.no/URN:NBN:no-66656|