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dc.date.accessioned2018-11-09T08:01:06Z
dc.date.available2018-11-09T08:01:06Z
dc.date.created2017-06-11T13:47:05Z
dc.date.issued2017
dc.identifier.citationVeldman, Sarina Gravendeel, Barbara Otieno, Joseph Lammers, Youri Duijm, Elza Nieman, Aline Bytebier, Benny Ngugi, Grace Martos, Florent van Andel, TR de Boer, Hugo . High-throughput sequencing of African chikanda cake highlights conservation challenges in orchids. Biodiversity and Conservation. 2017
dc.identifier.urihttp://hdl.handle.net/10852/65441
dc.description.abstractChikanda is a traditional dish made with wild-harvested ground orchid tubers belonging to three orchidioid genera, Disa, Satyrium and Habenaria, all of which are CITES appendix II-listed. Identification of collected orchid tubers is very difficult and documentation of constituent species in prepared chikanda has hitherto been impossible. Here amplicon metabarcoding was used in samples of six prepared chikanda cakes to study genetic sequence diversity and species diversity in this product. Molecular operational taxonomic unit identification using similarity-matching reveals that species of all three genera were present in the chikanda samples studied. Disa was present in all of the samples, Satyrium in five out of six and Habenaria in one of the samples, as well as a number of other plants. The fact that each sample contained orchids and the presence of a wide variety of species from all genera in this traditional dish raise serious concerns about the sustainability of this trade and the future of wild orchid populations in the main harvest areas. This proof-of-concept study shows that Ion-Torrent PGM is a cost-effective scalable platform for metabarcoding using the relatively long nrITS1 and nrITS2 regions. Furthermore, nrITS metabarcoding can be successfully used for the detection of specific ingredients in a highly-processed food product at genus level, and this makes it a useful tool in the detection of possible conservation issues arising from commercialized trade or processed plant products.en_US
dc.languageEN
dc.language.isoenen_US
dc.publisherKluwer Academic/Plenum Publishers
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleHigh-throughput sequencing of African chikanda cake highlights conservation challenges in orchidsen_US
dc.typeJournal articleen_US
dc.creator.authorVeldman, Sarina
dc.creator.authorGravendeel, Barbara
dc.creator.authorOtieno, Joseph
dc.creator.authorLammers, Youri
dc.creator.authorDuijm, Elza
dc.creator.authorNieman, Aline
dc.creator.authorBytebier, Benny
dc.creator.authorNgugi, Grace
dc.creator.authorMartos, Florent
dc.creator.authorvan Andel, TR
dc.creator.authorde Boer, Hugo
cristin.unitcode185,28,0,0
cristin.unitnameNaturhistorisk museum
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1
dc.identifier.cristin1475158
dc.identifier.bibliographiccitationinfo:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Biodiversity and Conservation&rft.volume=&rft.spage=&rft.date=2017
dc.identifier.jtitleBiodiversity and Conservation
dc.identifier.volume26
dc.identifier.issue9
dc.identifier.startpage2029
dc.identifier.endpage2046
dc.identifier.doihttp://dx.doi.org/10.1007/s10531-017-1343-7
dc.identifier.urnURN:NBN:no-67869
dc.type.documentTidsskriftartikkelen_US
dc.type.peerreviewedPeer reviewed
dc.source.issn0960-3115
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/65441/1/Veldman%2Bet%2Bal_2017_BIOC.pdf
dc.type.versionPublishedVersion


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