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dc.date.accessioned2018-09-12T09:25:36Z
dc.date.available2018-09-12T09:25:36Z
dc.date.created2017-07-04T21:40:42Z
dc.date.issued2017
dc.identifier.citationVehus, Tore Waaler, Jo Krauss, Stefan Lundanes, Elsa Wilson, Steven Ray Haakon . Combining HIC, SEC, and IEX with Fluorescence Polarization for Drug Target Discovery. LC GC North America. 2017, 30(5), 232-239
dc.identifier.urihttp://hdl.handle.net/10852/64645
dc.description.abstractFluorescence polarization (FP) is a highly regarded technique for studying drug–protein interactions, but has limited value regarding protein mixtures. As a novel approach to drug target discovery, the possibility of combining FP with liquid chromatography (LC) was explored. Nondenaturing protein LC principles such as size-exclusion chromatography (SEC), hydrophobic interaction chromatography (HIC), and ion exchange chromatography (IEX) were found to be orthogonal and compatible with FP because the mobile phases used do not negatively affect detection. For simple protein mixtures, the SEC/HIC/IEX–FP approach was able to identify tankyrase as the target of a triazole-based inhibitor of the Wnt signaling pathway, which is heavily associated with colon cancer. However, the total peak capacity of the three LC dimensions was not sufficient to resolve at cell-proteome level, calling for higher resolution of intact proteins to enable stand-alone drug target discovery with LC and FP.en_US
dc.languageEN
dc.titleCombining HIC, SEC, and IEX with Fluorescence Polarization for Drug Target Discoveryen_US
dc.typeJournal articleen_US
dc.creator.authorVehus, Tore
dc.creator.authorWaaler, Jo
dc.creator.authorKrauss, Stefan
dc.creator.authorLundanes, Elsa
dc.creator.authorWilson, Steven Ray Haakon
cristin.unitcode185,15,12,63
cristin.unitnameSeksjon for kjemisk livsvitenskap - biomolekyler, bio-inspirerte materialer og bioanalytisk kjemi
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1
dc.identifier.cristin1480883
dc.identifier.bibliographiccitationinfo:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=LC GC North America&rft.volume=30&rft.spage=232&rft.date=2017
dc.identifier.jtitleLC GC North America
dc.identifier.volume30
dc.identifier.issue5
dc.identifier.startpage232
dc.identifier.endpage239
dc.identifier.urnURN:NBN:no-67181
dc.type.documentTidsskriftartikkelen_US
dc.type.peerreviewedPeer reviewed
dc.source.issn1527-5949
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/64645/2/2017_LC-GC_Europe_Vehus.pdf
dc.type.versionPublishedVersion


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