Hide metadata

dc.date.accessioned2018-08-25T13:30:01Z
dc.date.available2018-08-25T13:30:01Z
dc.date.created2017-08-30T17:04:06Z
dc.date.issued2017
dc.identifier.citationHassan, Karl A. Fagerlund, Annette Elbourne, Liam D.H. Vörös, Aniko Kroeger, Jasmin Kaori Simm, Roger Tourasse, Nicolas Jacques Finke, Sarah Henderson, Peter J.F. Økstad, Ole Andreas Løchen Paulsen, Ian T. Kolstø, Anne-Brit . The putative drug efflux systems of the Bacillus cereus group. PLoS ONE. 2017, 12:e0176188(5), 1-25
dc.identifier.urihttp://hdl.handle.net/10852/63767
dc.description.abstractThe Bacillus cereus group of bacteria includes seven closely related species, three of which, B. anthracis, B. cereus and B. thuringiensis, are pathogens of humans, animals and/or insects. Preliminary investigations into the transport capabilities of different bacterial lineages suggested that genes encoding putative efflux systems were unusually abundant in the B. cereus group compared to other bacteria. To explore the drug efflux potential of the B. cereus group all putative efflux systems were identified in the genomes of prototypical strains of B. cereus, B. anthracis and B. thuringiensis using our Transporter Automated Annotation Pipeline. More than 90 putative drug efflux systems were found within each of these strains, accounting for up to 2.7% of their protein coding potential. Comparative analyses demonstrated that the efflux systems are highly conserved between these species; 70–80% of the putative efflux pumps were shared between all three strains studied. Furthermore, 82% of the putative efflux system proteins encoded by the prototypical B. cereus strain ATCC 14579 (type strain) were found to be conserved in at least 80% of 169 B. cereus group strains that have high quality genome sequences available. However, only a handful of these efflux pumps have been functionally characterized. Deletion of individual efflux pump genes from B. cereus typically had little impact to drug resistance phenotypes or the general fitness of the strains, possibly because of the large numbers of alternative efflux systems that may have overlapping substrate specificities. Therefore, to gain insight into the possible transport functions of efflux systems in B. cereus, we undertook large-scale qRT-PCR analyses of efflux pump gene expression following drug shocks and other stress treatments. Clustering of gene expression changes identified several groups of similarly regulated systems that may have overlapping drug resistance functions. In this article we review current knowledge of the small molecule efflux pumps encoded by the B. cereus group and suggest the likely functions of numerous uncharacterised pumps.en_US
dc.languageEN
dc.publisherPublic Library of Science (PLoS)
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleThe putative drug efflux systems of the Bacillus cereus groupen_US
dc.typeJournal articleen_US
dc.creator.authorHassan, Karl A.
dc.creator.authorFagerlund, Annette
dc.creator.authorElbourne, Liam D.H.
dc.creator.authorVörös, Aniko
dc.creator.authorKroeger, Jasmin Kaori
dc.creator.authorSimm, Roger
dc.creator.authorTourasse, Nicolas Jacques
dc.creator.authorFinke, Sarah
dc.creator.authorHenderson, Peter J.F.
dc.creator.authorØkstad, Ole Andreas Løchen
dc.creator.authorPaulsen, Ian T.
dc.creator.authorKolstø, Anne-Brit
cristin.unitcode185,15,23,0
cristin.unitnameFarmasøytisk institutt
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1
dc.identifier.cristin1490030
dc.identifier.bibliographiccitationinfo:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=PLoS ONE&rft.volume=12:e0176188&rft.spage=1&rft.date=2017
dc.identifier.jtitlePLoS ONE
dc.identifier.volume12:e0176188
dc.identifier.issue5
dc.identifier.startpage1
dc.identifier.endpage25
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0176188
dc.identifier.urnURN:NBN:no-66315
dc.type.documentTidsskriftartikkelen_US
dc.type.peerreviewedPeer reviewed
dc.source.issn1932-6203
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/63767/4/journal.pone.0176188.pdf
dc.type.versionPublishedVersion
dc.relation.projectNOFIMA/201704
dc.relation.projectNFR/262306


Files in this item

Appears in the following Collection

Hide metadata

Attribution 4.0 International
This item's license is: Attribution 4.0 International