Background: In 2016 the report from World Tourism Organization revealed an increase in international tourism for seven consecutive years, reaching 1,2 billion travellers. The highest increase was seen in Asia, Africa and the Pacific. The high prevalence of multidrug resistant microbes imposes a great risk of infection and/or colonization for travellers. Use of antibiotics is regarded as one of the major risk factors for being colonized by ESBL-producing enterobacteriaceae. Objective: Mapping the use and attitude towards antibiotics among western tourists travelling in Southeast Asia. Methods: Questionnaire among western tourists at Tan Son Nhat international airport Ho Chi Minh City, and Suvarnabhumi international airport Bangkok. Results: Our study included 191 tourists, among which 23,6 % reported having been in possession of antibiotics during their travels, and 13,3 % reported having used antibiotics. The majority got their antibiotics from a doctor in their home country, and in this group 52 % reported using it during the journey. In total, 3,7 % reported having used antibiotics to treat traveller’s diarrhoea. Internet and pharmacies were the most common sources for advice in case of illness. Conclusion: Western tourists consume an alarmingly high amount of antibiotics during travels to Southeast Asia, which increases the risk of colonization by ESBL-producing enterobacteriaceae. Action should be made to prevent the spread of multidrug resistant microbes from popular tourist destinations with a high prevalence of antibiotic resistance.