Raising the bar - Molecular identification of plants
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- Kulturhistorisk museum [1908]
Abstract
Up to a third of biodiversity is threatened due to the global economy and its international supply chains. The lack of global assessments of international supply chains and appropriate methods for the tractability of the traded products complicate the evaluation of the international trade’s impact on biodiversity loss. The goal of this thesis is, first, to explore new tech- nologies for traditional barcoding and metabarcoding, and then to develop alternative approaches to achieve better resolu- tion for the identification of species. Paper I shows illegal plant trade of orchids using standard barcoding markers and distance-based methods to identify traded species. These tools are also used in Paper II, which aims to identify adulteration and substitution of Hypericum perforatum L. herbal supplements. Paper III focuses on ginseng. This paper uses species delimitation approaches based on the phylogenomics of plastid genomes for the identification of ginseng species. Paper IV presents the evolution of Anacyclus and tests for hybridization using hundreds of nuclear genes. Then, in Paper V, traded market samples are used to understand the supply chain of Anacyclus pyrethrum.List of papers
Article I: De Boer, H.J., Ghorbani, A., Manzanilla, V., Raclariu, A.C., Kreziou, A., Ounjai, S., Osathanunkul, M., Gravendeel, B. 2017. DNA metabarcoding of orchid-derived products reveals widespread illegal orchid trade. Proc. R. Soc. B. 284: 20171182. DOI: 10.1098/rspb.2017.1182 The published version is not available in DUO due to publisher restrictions. The accepted version is available in DUO: http://urn.nb.no/URN:NBN:no-62818 |
Article II: Raclariu, A.C., Paltinean, R., Vlase, L., Labarre, A., Manzanilla, V., Ichim, M.C., Crisan, G., Brysting, A.K., de Boer, H.J. 2017. Comparative authentication of Hypericum perforatum herbal products using DNA metabarcoding, TLC and HPLC-MS. Sci. Rep. 7: 1291. DOI: 10.1038/s41598-017-01389-w The paper is included in the thesis. Also available in DUO: http://urn.nb.no/URN:NBN:no-62623 |
Article III: Manzanilla, V., Kool, A., Nguyen Nhat, L., Nong Van, H., Le Thi Thu, H., de Boer, H.J. 2018. Phylogenomics and barcoding of Panax: toward the identification of ginseng species. BMC Evolutionary Biology. 2018 Apr 03;18(1):44 The paper is included in the thesis. Also available in DUO: http://urn.nb.no/URN:NBN:no-64093 |
Article IV: Manzanilla, V., Ouhammou, A., Martin, G.J., Kolár, Schrøder-Nielsen, A., de Boer, H.J., Kool, A. Hybridisation and adaptive radiation of the genus Anacyclus L. (Anthemidae, Compositae). In prep. To be published. The paper is not available in DUO awaiting publishing. |
Article V: Manzanilla, V., Teixidor-Toneu, I., Martin, G.J., de Boer, H.J., Kool, A. Raising the bar of molecular plant identification sheds light on complex trade in red-listed species. In prep. To be published. The paper is not available in DUO awaiting publishing. |