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dc.date.accessioned2018-06-07T15:02:25Z
dc.date.available2018-06-07T15:02:25Z
dc.date.created2017-01-25T10:21:12Z
dc.date.issued2017
dc.identifier.citationMartîn, Ruth Martîn Portantier, Marina Chica-Balaguera, Nathalia Nyquist-Andersen, Mari Mata, J Lopez-Aviles, Sandra . A PP2A-B55-Mediated Crosstalk between TORC1 and TORC2 Regulates the Differentiation Response in Fission Yeast. Current Biology. 2017, 27(2), 175-188
dc.identifier.urihttp://hdl.handle.net/10852/61816
dc.description.abstractExtracellular cues regulate cell fate, and this is mainly achieved through the engagement of specific transcriptional programs. The TORC1 and TORC2 complexes mediate the integration of nutritional cues to cellular behavior, but their interplay is poorly understood. Here, we use fission yeast to investigate how phosphatase activity participates in this interplay during the switch from proliferation to sexual differentiation. We find that loss of PP2A-B55Pab1 enhances the expression of differentiation-specific genes and leads to premature conjugation. pab1 deletion brings about a transcriptional profile similar to TORC1 inactivation, and deletion of pab1 overcomes the repression of differentiation genes in cells overexpressing TORC1. Importantly, we show that this effect is mediated by an increased TORC2-AKT (Gad8) signaling. Under nutrient-rich conditions, PP2A-B55Pab1 dephosphorylates Gad8 Ser546, repressing its activity. Conversely, TORC1 inactivation upon starvation leads to the inactivation of PP2A-B55Pab1 through the Greatwall-Endosulfin pathway. This results in the activation of Gad8 and the commitment to differentiation. Thus, PP2A-B55Pab1 enables a crosstalk between the two TOR complexes that controls cell-fate decisions in response to nutrient availability.en_US
dc.languageEN
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleA PP2A-B55-Mediated Crosstalk between TORC1 and TORC2 Regulates the Differentiation Response in Fission Yeasten_US
dc.typeJournal articleen_US
dc.creator.authorMartîn, Ruth Martîn
dc.creator.authorPortantier, Marina
dc.creator.authorChica-Balaguera, Nathalia
dc.creator.authorNyquist-Andersen, Mari
dc.creator.authorMata, J
dc.creator.authorLopez-Aviles, Sandra
cristin.unitcode185,57,0,0
cristin.unitnameNorsk Senter for Molekylærmedisin
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode2
dc.identifier.cristin1437192
dc.identifier.bibliographiccitationinfo:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Current Biology&rft.volume=27&rft.spage=175&rft.date=2017
dc.identifier.jtitleCurrent Biology
dc.identifier.volume27
dc.identifier.issue2
dc.identifier.startpage175
dc.identifier.endpage188
dc.identifier.doihttp://dx.doi.org/10.1016/j.cub.2016.11.037
dc.identifier.urnURN:NBN:no-64418
dc.type.documentTidsskriftartikkelen_US
dc.type.peerreviewedPeer reviewed
dc.source.issn0960-9822
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/61816/1/PIIS0960982216313902.pdf
dc.type.versionPublishedVersion
dc.relation.projectNFR/187615


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