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dc.date.accessioned2018-04-27T07:35:38Z
dc.date.available2018-04-27T07:35:38Z
dc.date.issued2014
dc.identifier.urihttp://hdl.handle.net/10852/61613
dc.description.abstractIso-octyl chain-hydroxylated oxysterols were determined in attomoles per 10,000 cells concentrations in 10,000–80,000 cultured pancreatic adenocarcinoma cells, using a sensitive, highly automated nano-LC-ESI-MS-based method. Identified oxysterols included 24S hydroxycholesterol (24S-OHC), 25 hydroxycholesterol (25-OHC), and 27 hydroxycholesterol (27-OHC), while 20S hydroxycholesterol and 22S hydroxycholesterol were not detected. Lower mass limit of quantification was 23 fg (65 amol) for 25-OHC and 27-OHC (100 times lower than our previous method) and 54 fg (135 amol) for 24S-OHC, after derivatization into Girard T hydrazones and online sample cleanup using simplified and robust automatic filtration and filter back flushing solid phase extraction LC/MS/MS. The instrument configuration was easily installed using a commercial nano-LC/MS system. Recoveries in spiked sample were 96, 97, and 77% for 24S-OHC, 25-OHC, and 27-OHC, with within- and between-day repeatabilities of 1–21% and 2–20% relative SD, respectively. The study demonstrates the potential of nano-LC in lipidomics/sterolomics. This research was originally published in the Journal of Lipid Research. Roberg-Larsen H, Lund K, Vehus T, Solberg N, Vesterdal C, Misaghian D, Olsen PA, Krauss S, Wilson SR, Lundanes E.. Highly automated nano-LC/MS-based approach for thousand cell-scale quantification of side chain-hydroxylated oxysterols. J. Lipid Res. 2014 Jul;55(7):1531-6. © the American Society for Biochemistry and Molecular Biology.en_US
dc.language.isoenen_US
dc.relation.ispartofRøberg-Larsen, Hanne (2016) High sensitivity measurements of side-chain oxysterols using liquid chromatography mass spectrometry. Doctoral thesis. http://hdl.handle.net/10852/61614
dc.relation.urihttp://hdl.handle.net/10852/61614
dc.titleHighly automated nano-LC/MS-based approach for thousand cell-scale quantification of side chain-hydroxylated oxysterols[S]en_US
dc.typeJournal articleen_US
dc.creator.authorRøberg-Larsen, Hanne
dc.creator.authorLund, Kaja
dc.creator.authorVehus, Tore
dc.creator.authorHoen, Nina
dc.creator.authorVesterdal, Caroline
dc.creator.authorMisaghian, Dorna
dc.creator.authorOlsen, Peter Angell
dc.creator.authorKrauss, Stefan
dc.creator.authorWilson, Steven Ray Haakon
dc.creator.authorLundanes, Elsa
dc.identifier.jtitleJournal of Lipid Research
dc.identifier.volume55
dc.identifier.startpage1531
dc.identifier.endpage1536
dc.identifier.doihttps://doi.org/10.1194/jlr.D048801
dc.identifier.urnURN:NBN:no-64221
dc.type.documentTidsskriftartikkelen_US
dc.type.peerreviewedPeer reviewed
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/61613/1/J-Lipid-Res-2014-Roberg-Larsen-1531-6.pdf
dc.type.versionPublishedVersion


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