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dc.date.accessioned2018-02-21T15:44:41Z
dc.date.available2018-02-21T15:44:41Z
dc.date.issued2018
dc.identifier.urihttp://hdl.handle.net/10852/60289
dc.description.abstractThe results presented in this thesis describe the investigation of Piscirickettsia salmonis derived membrane vesicles (MVs) as a vaccine candidate against salmonid rickettsial septicemia. MVs are small spherical structures known to contain a variety of bacterial components, including proteins, LPS, DNA and RNA, which makes the vesicles similar to their mother cell in many aspects. Comparative characterization of MVs from three different isolates of the bacterium revealed several strain specific differences, both in vivo and in vitro. When used for immunization in an adult zebrafish model the vesicles induced a protective response against P. salmonis. Incorporation of chitosan as an adjuvant, by MV encapsulation, did furthermore enhance the protective effect of the vesicles in adult zebrafish. Histological analysis indicated a reduced bacterial load upon challenge in the MV immunized group, and the RNA expression of several immune related genes altered, including mpeg1.1, tnfα, il1b, il10 and il6. MVs induced the secretion of IgM upon immunization, suggesting an immunogenic effect of the vesicles. Taken together, the data indicate a vaccine potential of MVs against SRS.en_US
dc.language.isoenen_US
dc.relation.haspartPaper I: Comparative analysis of membrane vesicles from three Piscirickettsia salmonis isolates reveals differences in vesicle characteristics. Tandberg JI, Lagos LX, Langlete P, Berger E, Rishovd AL, Roos N, Varkey D, Paulsen IT, Winther-Larsen HC. PLoS One, 2016 Oct 20; 11(10):e0165099. The article is included in the thesis. Also available at http://urn.nb.no/URN:NBN:no-63032
dc.relation.haspartPaper II: Membrane vesicles from Piscirickettsia salmonis induce protective immunity and reduce development of salmonid rickettsial septicemia in an adult zebrafish model. Tandberg JI, Oliver C, Lagos LX, Gaarder M, Yáñez A, Ropstad E, Winther-Larsen HC. Fish and Shellfish Immunology, 2017 Jun 6; pii: S1050-4648(17)30336-4. The article is included in the thesis. Also available at http://urn.nb.no/URN:NBN:no-62950
dc.relation.haspartPaper III: Use of chitosan coated membrane vesicles for immunization against salmonid rickettsial septicemia in an adult zebrafish model. Tandberg JI, Lagos LX, Ropstad E, Smistad G, Hiorth M, Winther-Larsen HC. Manuscript. To be published. The paper is not available in DUO awaiting publishing.
dc.relation.urihttp://urn.nb.no/URN:NBN:no-63032
dc.relation.urihttp://urn.nb.no/URN:NBN:no-62950
dc.titleCharacterization of Piscirickettsia salmonis membrane vesicles and their use as a vaccine for aquacultureen_US
dc.typeDoctoral thesisen_US
dc.creator.authorTandberg, Julia Isabel
dc.identifier.urnURN:NBN:no-62947
dc.type.documentDoktoravhandlingen_US
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/60289/1/PhD-Julia-Isabel-Tandberg-2018.pdf


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