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dc.date.accessioned2018-02-12T11:38:46Z
dc.date.available2018-02-12T11:38:46Z
dc.date.created2017-12-04T13:14:50Z
dc.date.issued2017
dc.identifier.citationGregersen, Ida Sandanger, Øystein Askevold, Erik Tandberg Sagen, Ellen Lund Yang, Kuan Holm, Sverre Pedersen, Turid Margrethe Skjelland, Mona Krohg-Sørensen, Kirsten Hansen, Trond Vidar Dahl, Tuva Børresdatter Otterdal, Kari Espevik, Terje Aukrust, Pål Yndestad, Arne Halvorsen, Bente . Interleukin 27 is increased in carotid atherosclerosis and promotes NLRP3 inflammasome activation.. PLoS ONE. 2017
dc.identifier.urihttp://hdl.handle.net/10852/60035
dc.description.abstractAim: Interleukin-27 (IL-27) is involved in different inflammatory diseases; however, its role in atherosclerosis is unclear. In this study we investigated the expression of IL-27 and its receptor in patients with carotid atherosclerosis and if IL-27 could modulate the inflammatory effects of the NLRP3 inflammasome in vitro. Methods: Plasma IL-27 was measured by enzyme immunoassay in patients with carotid stenosis (n = 140) and in healthy controls (n = 19). Expression of IL-27 and IL-27R was analyzed by quantitative PCR and immunohistochemistry in plaques from patients and in non-atherosclerotic vessels. THP-1 monocytes, primary monocytes and peripheral blood mononuclear cells (PBMCs) were used to study effects of IL-27 in vitro. Results: Our main findings were: (i) Plasma levels of IL-27 were significantly elevated in patients with carotid atherosclerotic disease compared to healthy controls. (ii) Gene expression of IL-27 and IL-27R was significantly elevated in plaques compared to control vessels, and co-localized to macrophages. (iii) In vitro, IL-27 increased NLRP3 inflammasome activation in monocytes with enhanced release of IL-1 β. Conclusions: We demonstrate increased levels of IL-27 and IL-27R in patients with carotid atherosclerosis. Our in vitro findings suggest an inflammatory role for IL-27, which can possibly be linked to atherosclerotic disease development.en_US
dc.languageEN
dc.publisherPublic Library of Science (PLoS)
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleInterleukin 27 is increased in carotid atherosclerosis and promotes NLRP3 inflammasome activation.en_US
dc.typeJournal articleen_US
dc.creator.authorGregersen, Ida
dc.creator.authorSandanger, Øystein
dc.creator.authorAskevold, Erik Tandberg
dc.creator.authorSagen, Ellen Lund
dc.creator.authorYang, Kuan
dc.creator.authorHolm, Sverre
dc.creator.authorPedersen, Turid Margrethe
dc.creator.authorSkjelland, Mona
dc.creator.authorKrohg-Sørensen, Kirsten
dc.creator.authorHansen, Trond Vidar
dc.creator.authorDahl, Tuva Børresdatter
dc.creator.authorOtterdal, Kari
dc.creator.authorEspevik, Terje
dc.creator.authorAukrust, Pål
dc.creator.authorYndestad, Arne
dc.creator.authorHalvorsen, Bente
cristin.unitcode185,53,48,14
cristin.unitnameInstitutt for indremedisinsk forskning
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1
dc.identifier.cristin1522499
dc.identifier.bibliographiccitationinfo:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=PLoS ONE&rft.volume=&rft.spage=&rft.date=2017
dc.identifier.jtitlePLoS ONE
dc.identifier.doi10.1371/journal.pone.0188387
dc.identifier.urnURN:NBN:no-62705
dc.type.documentTidsskriftartikkelen_US
dc.type.peerreviewedPeer reviewed
dc.source.issn1932-6203
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/60035/2/Interleukin%2B27%2Bis%2Bincreased%2Bin%2Bcarotid%2Batherosclerosis%2Band%2Bpromotes%2BNLRP3%2Binflammasome%2Bactivation.pdf
dc.type.versionPublishedVersion


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