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dc.date.accessioned2018-02-08T08:11:45Z
dc.date.available2018-02-08T08:11:45Z
dc.date.created2017-06-11T13:51:05Z
dc.date.issued2017
dc.identifier.citationRaclariu, Ancuta Cristina Mocan, Andrei Popa, Madalina Oana Vlase, Laurian Ichim, Mihael Cristi Crisan, Gianina Brysting, Anne Krag de Boer, Hugo . Veronica officinalis product authentication using DNA metabarcoding and HPLC-MS reveals widespread adulteration with Veronica chamaedrys. Frontiers in Pharmacology. 2017, 8, 1-13
dc.identifier.urihttp://hdl.handle.net/10852/59947
dc.description.abstractStudying herbal products derived from local and traditional knowledge and their value chains is one of the main challenges in ethnopharmacology. The majority of these products have a long history of use, but non-harmonized trade and differences in regulatory policies between countries impact their value chains and lead to concerns over product efficacy, safety and quality. Veronica officinalis L. (common speedwell), a member of Plantaginaceae family, has a long history of use in European traditional medicine, mainly in central eastern Europe and the Balkans. However, no specified control tests are available either to establish the quality of derived herbal products or for the discrimination of its most common substitute, V. chamaedrys L. (germander speedwell). In this study, we use DNA metabarcoding and high performance liquid chromatography coupled with mass spectrometry (HPLC-MS) to authenticate sixteen V. officinalis herbal products and compare the potential of the two approaches to detect substitution, adulteration and the use of unreported constituents. HPLC-MS showed high resolution in detecting phytochemical target compounds, but did not enable detection of specific plant species in the products. DNA metabarcoding detected V. officinalis in only 15% of the products, whereas it detected V. chamaedrys in 62% of the products. The results confirm that DNA metabarcoding can be used to test for the presence of Veronica species, and detect substitution and/or admixture of other Veronica species, as well as simultaneously detect all other species present. Our results confirm that none of the herbal products contained exactly the species listed on the label, and all included substitutes, contaminants or fillers. This study highlights the need for authentication of raw herbals along the value chain of these products. An integrative methodology can assess both the quality of herbal products in terms of target compound concentrations and species composition, as well as admixture and substitution with other chemical compounds and plants.en_US
dc.languageEN
dc.language.isoenen_US
dc.publisherFrontiers Media
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleVeronica officinalis product authentication using DNA metabarcoding and HPLC-MS reveals widespread adulteration with Veronica chamaedrysen_US
dc.typeJournal articleen_US
dc.creator.authorRaclariu, Ancuta Cristina
dc.creator.authorMocan, Andrei
dc.creator.authorPopa, Madalina Oana
dc.creator.authorVlase, Laurian
dc.creator.authorIchim, Mihael Cristi
dc.creator.authorCrisan, Gianina
dc.creator.authorBrysting, Anne Krag
dc.creator.authorde Boer, Hugo
cristin.unitcode185,28,8,5
cristin.unitnameForskningsgruppen Planteevolusjon og DNA Metabarcoding
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1
dc.identifier.cristin1475159
dc.identifier.bibliographiccitationinfo:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Frontiers in Pharmacology&rft.volume=8&rft.spage=1&rft.date=2017
dc.identifier.jtitleFrontiers in Pharmacology
dc.identifier.volume8
dc.identifier.startpage1
dc.identifier.endpage13
dc.identifier.doihttp://dx.doi.org/10.3389/fphar.2017.00378
dc.identifier.urnURN:NBN:no-62617
dc.type.documentTidsskriftartikkelen_US
dc.type.peerreviewedPeer reviewed
dc.source.issn1663-9812
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/59947/1/Raclariu_2017_Veronica.pdf
dc.type.versionPublishedVersion
cristin.articleid378
dc.relation.projectNFR/179569


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