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dc.date.accessioned2017-10-27T06:54:57Z
dc.date.available2017-10-27T06:54:57Z
dc.date.created2010-01-14T10:06:10Z
dc.date.issued2009
dc.identifier.citationSivertsen, T Nygaard, AK Mathisen, Gro Haarklou Fonnum, Frode . Effects of 4-methylimidazole on cerebral glutamate decarboxylase activity and specific GABA receptor binding in mice. Toxicology Mechanisms and Methods. 2009, 19(3), 214-218
dc.identifier.urihttp://hdl.handle.net/10852/58968
dc.description.abstract4-Methylimidazole (4MeI) is a tremorogenic and convulsive agent of concern both in human and veterinary toxicology. The in vitro effects of 4MeI (5 μM–20 mM) on cerebral glutamate decarboxylase (GAD) activity and (in concentrations up to 50 mM) on binding of [3H]GABA to cerebral GABA receptors were tested in brain tissue from B6D2 mice. The effects of 1-methylimidazole (1MeI), 2-methylimidazole (2MeI), 4-methylhydroxy-imidazole (4MeOHI), imidazole-4-acetic acid (4AcI) (all in concentrations of 5–20 mM) and imidazole (20 mM) on GAD activity were also tested. In addition, the effect of a lethal dose of 4MeI (250 mg/kg ip) to B6D2 mice in vivo on the postmortem concentrations of γ-aminobutyric acid (GABA) and glutamate in their brains were measured. In all experiments, student’s t-test was used for statistical comparison. 4MeI in concentrations of 2 mM and above did inhibit GAD activity significantly in vitro, but glutamate and GABA concentrations in mouse brains after lethal 4MeI poisoning were not significantly different from control values. The effect of 2MeI on GAD activity was stronger than the effect of 4MeI. Binding of [3H]GABA to cerebral GABA receptors in vitro was significantly inhibited only at 4MeI concentrations of 5 mM and above. The results indicate that neither inhibition of GABA synthesis nor competitive inhibition of the binding of GABA to its receptors are likely mechanisms for the excitation and convulsions seen in 4MeI poisoning in animals.en_US
dc.languageEN
dc.language.isoenen_US
dc.publisherTaylor & Francis
dc.rightsAttribution 3.0 Unported
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/
dc.titleEffects of 4-methylimidazole on cerebral glutamate decarboxylase activity and specific GABA receptor binding in miceen_US
dc.typeJournal articleen_US
dc.creator.authorSivertsen, T
dc.creator.authorNygaard, AK
dc.creator.authorMathisen, Gro Haarklou
dc.creator.authorFonnum, Frode
cristin.unitcode185,15,23,30
cristin.unitnameFarmasøytisk biovitenskap
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1
dc.identifier.cristin342706
dc.identifier.bibliographiccitationinfo:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Toxicology Mechanisms and Methods&rft.volume=19&rft.spage=214&rft.date=2009
dc.identifier.jtitleToxicology Mechanisms and Methods
dc.identifier.volume19
dc.identifier.issue3
dc.identifier.startpage214
dc.identifier.endpage218
dc.identifier.doihttp://dx.doi.org/10.1080/15376510802488173
dc.identifier.urnURN:NBN:no-61429
dc.type.documentTidsskriftartikkelen_US
dc.type.peerreviewedPeer reviewed
dc.source.issn1537-6516
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/58968/2/342706.pdf
dc.type.versionPublishedVersion


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