Background: Research investigating potential brain structural differences between depressed cohorts and healthy controls have found significant volumetric differences localised to the hippocampus. Specifically, studies have shown a reduction in volume in subregions of the hippocampus in sufferers of major depressive disorder (MDD). In animal studies, antidepressants have been implicated as potentially acting to counter hippocampal volume loss and aiding neurotrophic effects, but findings from human studies are sparse. Research aims: The current cross sectional study aimed to investigate hippocampal subfield volume differences between adult remitted MDD (rMDD) subjects and healthy controls. Moreover, the study investigated whether antidepressants had a neurotrophic effect on hippocampal subfield volumes. Method: Magnetic Resonance Imaging (MRI) data of 191 rMDD subjects and 77 healthy controls were processed using a new automated segmentation tool, implemented in FreeSurfer 6.0, for hippocampal segmentation. Results: Our analysis revealed a main effect across hippocampal subfields, indicating smaller volumes present in rMDD subjects localised to the granular cells of the dentate gyrus (GC_DG), CA4, parasubiculum, and molecular layer (ML), while larger subfield volumes were present in the presubiculum bilaterally. The current paper also demonstrates hippocampal subfield volume differences associated with medication status. Specifically, the findings revealed a main effect in previously medicated rMDD subjects, with hippocampal subfield volume observed as larger in the rMDD group. Conclusions: The study adds to the literature suggesting that hippocampal volume reductions are present in individuals with a history of depression. The results implicate the use of antidepressant drugs as neurotrophically enhancing hippocampal subfield volume, potentially partly through reversing neurogenesis suppression. Longitudinal studies are needed to determine any direct effects of antidepressants on hippocampal subfield volumes.