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dc.date.accessioned2017-08-04T10:58:43Z
dc.date.available2017-08-04T10:58:43Z
dc.date.created2015-01-23T15:27:48Z
dc.date.issued2014
dc.identifier.citationÖzdemir, BC Hensel, J Secondini, C Wetterwald, A Schwaninger, R Fleischmann, A Raffelsberger, W Poch, O Delorenzi, M Temanni, R Mills, Ian Geoffrey van der Pluijm, G Thalmann, GN Cecchini, MG . The Molecular Signature of the Stroma Response in Prostate Cancer-Induced Osteoblastic Bone Metastasis Highlights Expansion of Hematopoietic and Prostate Epithelial Stem Cell Niches. PLoS ONE. 2014, 9(12)
dc.identifier.urihttp://hdl.handle.net/10852/56776
dc.description.abstractThe reciprocal interaction between cancer cells and the tissue-specific stroma is critical for primary and metastatic tumor growth progression. Prostate cancer cells colonize preferentially bone (osteotropism), where they alter the physiological balance between osteoblast-mediated bone formation and osteoclast-mediated bone resorption, and elicit prevalently an osteoblastic response (osteoinduction). The molecular cues provided by osteoblasts for the survival and growth of bone metastatic prostate cancer cells are largely unknown. We exploited the sufficient divergence between human and mouse RNA sequences together with redefinition of highly species-specific gene arrays by computer-aided and experimental exclusion of cross-hybridizing oligonucleotide probes. This strategy allowed the dissection of the stroma (mouse) from the cancer cell (human) transcriptome in bone metastasis xenograft models of human osteoinductive prostate cancer cells (VCaP and C4-2B). As a result, we generated the osteoblastic bone metastasis-associated stroma transcriptome (OB-BMST). Subtraction of genes shared by inflammation, wound healing and desmoplastic responses, and by the tissue type-independent stroma responses to a variety of non-osteotropic and osteotropic primary cancers generated a curated gene signature (“Core” OB-BMST) putatively representing the bone marrow/bone-specific stroma response to prostate cancer-induced, osteoblastic bone metastasis. The expression pattern of three representative Core OB-BMST genes (PTN, EPHA3 and FSCN1) seems to confirm the bone specificity of this response. A robust induction of genes involved in osteogenesis and angiogenesis dominates both the OB-BMST and Core OB-BMST. This translates in an amplification of hematopoietic and, remarkably, prostate epithelial stem cell niche components that may function as a self-reinforcing bone metastatic niche providing a growth support specific for osteoinductive prostate cancer cells. The induction of this combinatorial stem cell niche is a novel mechanism that may also explain cancer cell osteotropism and local interference with hematopoiesis (myelophthisis). Accordingly, these stem cell niche components may represent innovative therapeutic targets and/or serum biomarkers in osteoblastic bone metastasis.en_US
dc.languageEN
dc.publisherPublic Library of Science (PLoS)
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleThe Molecular Signature of the Stroma Response in Prostate Cancer-Induced Osteoblastic Bone Metastasis Highlights Expansion of Hematopoietic and Prostate Epithelial Stem Cell Nichesen_US
dc.typeJournal articleen_US
dc.creator.authorÖzdemir, BC
dc.creator.authorHensel, J
dc.creator.authorSecondini, C
dc.creator.authorWetterwald, A
dc.creator.authorSchwaninger, R
dc.creator.authorFleischmann, A
dc.creator.authorRaffelsberger, W
dc.creator.authorPoch, O
dc.creator.authorDelorenzi, M
dc.creator.authorTemanni, R
dc.creator.authorMills, Ian Geoffrey
dc.creator.authorvan der Pluijm, G
dc.creator.authorThalmann, GN
dc.creator.authorCecchini, MG
cristin.unitcode185,29,22,0
cristin.unitnameCentre for Molecular Medicine Norway - Nordic EMBL Partnership
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1
dc.identifier.cristin1206039
dc.identifier.bibliographiccitationinfo:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=PLoS ONE&rft.volume=9&rft.spage=&rft.date=2014
dc.identifier.jtitlePLoS ONE
dc.identifier.volume9
dc.identifier.issue12
dc.identifier.pagecount32
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0114530
dc.identifier.urnURN:NBN:no-59528
dc.type.documentTidsskriftartikkelen_US
dc.type.peerreviewedPeer reviewed
dc.source.issn1932-6203
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/56776/2/journal.pone.0114530.PDF
dc.type.versionPublishedVersion


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