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dc.date.accessioned2017-07-17T13:10:07Z
dc.date.available2017-07-17T13:10:07Z
dc.date.created2016-06-21T13:18:38Z
dc.date.issued2017
dc.identifier.citationCollins, Andrew Richard Balasubramanyam, Annangi Rubio, Laura Marcos, Ricard Dorn, Marco Merker, Carolin Estrela-Lopis, Irina Cimpan, Mihaela Roxana Ibrahim, Mohamed Cimpan, Emil Ostermann, Melanie Sauter, Alexander El Yamani, Naouale Shaposhnikov, Sergey Chevillard, Sylvie Paget, Vincent Grall, Romain Delic, Jozo Goñi- de-Cerio, Felipe Suarez-Merino, Blanca Fessard, Valérie Hogeveen, Kevin N Fjellsbø, Lise Maria Runden Pran, Elise Brzicova, Tana Topinka, Jan João Silva, Maria Leite, PE Ribeiro, AR Granjeiro, JM Grafström, Roland Prina-Mello, Adriele Dusinska, Maria . High throughput toxicity screening and intracellular detection of nanomaterials. Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology. 2017, 9(1), 1-30
dc.identifier.urihttp://hdl.handle.net/10852/56062
dc.description.abstractWith the growing numbers of nanomaterials (NMs), there is a great demand for rapid and reliable ways of testing NM safety—preferably using in vitro approaches, to avoid the ethical dilemmas associated with animal research. Data are needed for developing intelligent testing strategies for risk assessment of NMs, based on grouping and read-across approaches. The adoption of high throughput screening (HTS) and high content analysis (HCA) for NM toxicity testing allows the testing of numerous materials at different concentrations and on different types of cells, reduces the effect of inter-experimental variation, and makes substantial savings in time and cost. HTS/HCA approaches facilitate the classification of key biological indicators of NM-cell interactions. Validation of in vitro HTS tests is required, taking account of relevance to in vivo results. HTS/HCA approaches are needed to assess dose- and time-dependent toxicity, allowing prediction of in vivo adverse effects. Several HTS/HCA methods are being validated and applied for NM testing in the FP7 project NANoREG, including Label-free cellular screening of NM uptake, HCA, High throughput flow cytometry, Impedance-based monitoring, Multiplex analysis of secreted products, and genotoxicity methods—namely High throughput comet assay, High throughput in vitro micronucleus assay, and γH2AX assay. There are several technical challenges with HTS/HCA for NM testing, as toxicity screening needs to be coupled with characterization of NMs in exposure medium prior to the test; possible interference of NMs with HTS/HCA techniques is another concern. Advantages and challenges of HTS/HCA approaches in NM safety are discussed.en_US
dc.languageEN
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.titleHigh throughput toxicity screening and intracellular detection of nanomaterialsen_US
dc.typeJournal articleen_US
dc.creator.authorCollins, Andrew Richard
dc.creator.authorBalasubramanyam, Annangi
dc.creator.authorRubio, Laura
dc.creator.authorMarcos, Ricard
dc.creator.authorDorn, Marco
dc.creator.authorMerker, Carolin
dc.creator.authorEstrela-Lopis, Irina
dc.creator.authorCimpan, Mihaela Roxana
dc.creator.authorIbrahim, Mohamed
dc.creator.authorCimpan, Emil
dc.creator.authorOstermann, Melanie
dc.creator.authorSauter, Alexander
dc.creator.authorEl Yamani, Naouale
dc.creator.authorShaposhnikov, Sergey
dc.creator.authorChevillard, Sylvie
dc.creator.authorPaget, Vincent
dc.creator.authorGrall, Romain
dc.creator.authorDelic, Jozo
dc.creator.authorGoñi- de-Cerio, Felipe
dc.creator.authorSuarez-Merino, Blanca
dc.creator.authorFessard, Valérie
dc.creator.authorHogeveen, Kevin N
dc.creator.authorFjellsbø, Lise Maria
dc.creator.authorRunden Pran, Elise
dc.creator.authorBrzicova, Tana
dc.creator.authorTopinka, Jan
dc.creator.authorJoão Silva, Maria
dc.creator.authorLeite, PE
dc.creator.authorRibeiro, AR
dc.creator.authorGranjeiro, JM
dc.creator.authorGrafström, Roland
dc.creator.authorPrina-Mello, Adriele
dc.creator.authorDusinska, Maria
cristin.unitcode185,51,13,20
cristin.unitnameSeksjon for klinisk ernæring
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1
dc.identifier.cristin1362899
dc.identifier.bibliographiccitationinfo:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology&rft.volume=9&rft.spage=1&rft.date=2017
dc.identifier.jtitleWiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology
dc.identifier.volume9
dc.identifier.issue1
dc.identifier.startpage1
dc.identifier.endpage30
dc.identifier.doihttp://dx.doi.org/10.1002/wnan.1413
dc.identifier.urnURN:NBN:no-58802
dc.type.documentTidsskriftartikkelen_US
dc.type.peerreviewedPeer reviewed
dc.source.issn1939-5116
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/56062/1/Collins_et_al-2017-Wiley_Interdisciplinary_Reviews__Nanomedicine_and_Nanobiotechnology.pdf
dc.type.versionPublishedVersion
cristin.articleid9:e1413


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