dc.date.accessioned | 2017-07-12T13:32:45Z | |
dc.date.available | 2017-07-12T13:32:45Z | |
dc.date.issued | 2017 | |
dc.identifier.uri | http://hdl.handle.net/10852/55981 | |
dc.description.abstract | Faithful chromosome segregation during mitosis relies on a proofreading mechanism that monitors proper kinetochore-microtubule attachments. The spindle assembly checkpoint (SAC) is based on the concerted action of numerous components that maintain a repressive signal inhibiting transition into anaphase until all chromosomes are attached. Here we show that A-Kinase Anchoring Protein 95 (AKAP95) is necessary for proper SAC function. AKAP95-depleted HeLa cells show micronuclei formed from lagging chromosomes at mitosis. Using a BioID proximity-based proteomic screen, we identify the nuclear pore complex protein TPR as a novel AKAP95 binding partner. We show interaction between AKAP95 and TPR in mitosis, and an AKAP95-dependent enrichment of TPR in the spindle microtubule area in metaphase, then later in the spindle midzone area. AKAP95-depleted cells display faster prometaphase to anaphase transition, escape from nocodazole-induced mitotic arrest and show a partial delocalization from kinetochores of the SAC component MAD1. Our results demonstrate an involvement of AKAP95 in proper SAC function likely through its interaction with TPR. | en_US |
dc.language.iso | en | en_US |
dc.relation.ispartof | López-Soop, Graciela (2017) Study of the multifunctional nuclear scaffold protein. A kinase anchoring protein 95. Doctoral thesis. http://urn.nb.no/URN:NBN:no-58742 | |
dc.relation.uri | http://urn.nb.no/URN:NBN:no-58742 | |
dc.title | AKAP95 interacts with nucleoporin TPR in mitosis and is important for the spindle assembly checkpoint | en_US |
dc.type | Journal article | en_US |
dc.creator.author | López-Soop, Graciela | |
dc.creator.author | Rønningen, Torunn | |
dc.creator.author | Rogala, Agnieszka | |
dc.creator.author | Richartz, Nina | |
dc.creator.author | Blomhoff, Heidi Kiil | |
dc.creator.author | Thiede, Bernd | |
dc.creator.author | Collas, Philippe | |
dc.creator.author | Küntziger, Thomas | |
dc.identifier.cristin | 1461591 | |
dc.identifier.jtitle | Cell Cycle | |
dc.identifier.volume | 16 | |
dc.identifier.issue | 10 | |
dc.identifier.startpage | 947 | |
dc.identifier.endpage | 956 | |
dc.identifier.doi | http://dx.doi.org/10.1080/15384101.2017.1310350 | |
dc.identifier.urn | URN:NBN:no-58731 | |
dc.type.document | Tidsskriftartikkel | en_US |
dc.identifier.fulltext | Fulltext https://www.duo.uio.no/bitstream/handle/10852/55981/1/Lopez-Soop-et-al-17.pdf | |
dc.type.version | SubmittedVersion | |