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dc.date.accessioned2017-07-12T13:32:45Z
dc.date.available2017-07-12T13:32:45Z
dc.date.issued2017
dc.identifier.urihttp://hdl.handle.net/10852/55981
dc.description.abstractFaithful chromosome segregation during mitosis relies on a proofreading mechanism that monitors proper kinetochore-microtubule attachments. The spindle assembly checkpoint (SAC) is based on the concerted action of numerous components that maintain a repressive signal inhibiting transition into anaphase until all chromosomes are attached. Here we show that A-Kinase Anchoring Protein 95 (AKAP95) is necessary for proper SAC function. AKAP95-depleted HeLa cells show micronuclei formed from lagging chromosomes at mitosis. Using a BioID proximity-based proteomic screen, we identify the nuclear pore complex protein TPR as a novel AKAP95 binding partner. We show interaction between AKAP95 and TPR in mitosis, and an AKAP95-dependent enrichment of TPR in the spindle microtubule area in metaphase, then later in the spindle midzone area. AKAP95-depleted cells display faster prometaphase to anaphase transition, escape from nocodazole-induced mitotic arrest and show a partial delocalization from kinetochores of the SAC component MAD1. Our results demonstrate an involvement of AKAP95 in proper SAC function likely through its interaction with TPR.en_US
dc.language.isoenen_US
dc.relation.ispartofLópez-Soop, Graciela (2017) Study of the multifunctional nuclear scaffold protein. A kinase anchoring protein 95. Doctoral thesis. http://urn.nb.no/URN:NBN:no-58742
dc.relation.urihttp://urn.nb.no/URN:NBN:no-58742
dc.titleAKAP95 interacts with nucleoporin TPR in mitosis and is important for the spindle assembly checkpointen_US
dc.typeJournal articleen_US
dc.creator.authorLópez-Soop, Graciela
dc.creator.authorRønningen, Torunn
dc.creator.authorRogala, Agnieszka
dc.creator.authorRichartz, Nina
dc.creator.authorBlomhoff, Heidi Kiil
dc.creator.authorThiede, Bernd
dc.creator.authorCollas, Philippe
dc.creator.authorKüntziger, Thomas
dc.identifier.cristin1461591
dc.identifier.jtitleCell Cycle
dc.identifier.volume16
dc.identifier.issue10
dc.identifier.startpage947
dc.identifier.endpage956
dc.identifier.doihttp://dx.doi.org/10.1080/15384101.2017.1310350
dc.identifier.urnURN:NBN:no-58731
dc.type.documentTidsskriftartikkelen_US
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/55981/1/Lopez-Soop-et-al-17.pdf
dc.type.versionSubmittedVersion


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