Abstract
Around three decades ago, environmental chemists developed a new technique to identify pollutants present in complex environmental mixtures: effect-directed analysis (EDA). EDA uses chemical tools (for the extraction, fractionation, and analysis of samples) together with biological tools (as bioassays) to find the cause of an observed effect. Such studies can be very powerful but the extensive material, the high number of fractions generated, and the expertise required by such intensive study can be an obstacle. This thesis tested different techniques for each EDA steps and several EDA studies have been performed. The aim of the thesis is to propose ways to increase EDA throughput and to facilitate the use of bioassays and chemical fractionations and analyses together. For the thesis, two bioassays were further developed and different fractionation and analytical techniques were performed and compared. In conclusion a «generic EDA» protocol was suggested as well as further development opportunities for EDA.
List of papers
Paper I: Automated high-throughput in vitro screening of the acetylcholine esterase inhibiting potential of environmental samples, mixtures and single compounds (2016), Jean Froment, Knut Erik Tollefsen, Kevin V. Thomas, Ecotoxicology and Environmental Safety 130, 74-80. The paper is not available in DUO due to publisher restrictions. The published version is available at: https://doi.org/10.1016/j.ecoenv.2016.04.005 |
Paper II: Identification of petrogenic produced water components as acetylcholine esterase inhibitors (2016), Jean Froment, Katherine Langford, Knut Erik Tollefsen, Inger Lise N. Bråte, Steven J. Brooks, Kevin V. Thomas, Environmental Pollution 215, 18-26. The paper is not available in DUO due to publisher restrictions. The published version is available at: https://doi.org/10.1016/j.envpol.2016.05.004 |
Paper III: Miniaturization of a transthyretin binding assay using a fluorescent probe for high throughput screening of thyroid hormone disruption in environmental samples (2017), Xiyu Ouyang and Jean Froment, Pim Leonards, Guttorm Christensen, Knut Erik Tollefsen, Jacob de Boer, Kevin V. Thomas, Marja Lamoree. Chemosphere 171, 722-728. The article is available in DUO: http://urn.nb.no/URN:NBN:no-58744 |
Paper IV: Potential algal photosynthesis inhibitors in landfill leachate detected by enhance throughput effect-directed analysis, Jean Froment, Xiyu Ouyang, Sascha Sjollema, Harm van der Geest, Knut Erik Tollefsen, Pim Leonards, Marja Lamoree, Kevin V. Thomas. In preparation. The paper is not available in DUO awaiting publishing. |
Paper V: Prioritization of algal growth inhibitors in treated waste water using effect-directed analysis based on non-target screening techniques, Zuzana Tousova, and Jean Froment, Peter Oswald, Kevin V. Thomas, Knut Erik Tollefsen, Malcolm Reid, Katherine Langford, Klara Hilscherova, Jaroslav Slobodník. In preparation. The paper is not available in DUO awaiting publishing. |