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dc.date.accessioned2017-06-26T11:28:07Z
dc.date.available2017-06-26T11:28:07Z
dc.date.created2017-05-08T14:21:15Z
dc.date.issued2017
dc.identifier.citationFeruglio, Siri Kvale, Dag Dyrhol-Riise, Anne Ma . T Cell Responses and Regulation and the Impact of In Vitro IL-10 and TGF-beta Modulation During Treatment of Active Tuberculosis. Scandinavian Journal of Immunology. 2017, 85(2), 138-146
dc.identifier.urihttp://hdl.handle.net/10852/55708
dc.description.abstractMycobacterium tuberculosis (Mtb) is particularly challenging for the immune system being an intracellular pathogen, and a variety of T cell subpopulations are activated by the host defence mechanism. In this study, we investigated T cell responses and regulation in active TB patients with drug-sensitive Mtb (N = 18) during 24 weeks of efficient anti-TB therapy. T cell activation, differentiation, regulatory T cell (Treg) subsets, Mtb-induced T cell proliferation and in vitro IL10 and TGF-beta modulation were analysed by flow cytometry at baseline and after 8 and 24 weeks of therapy, while soluble cytokines in culture supernatants were analysed by a 9-plex Luminex assay. Successful treatment resulted in significantly reduced co-expression of HLA-DR/CD38 and PD-1/CD38 on both CD4(+) and CD8(+) T cells, while the fraction of CD4+ CD25 high CD127 low Tregs (P = 0.017) and CD4(+) CD25(high) CD127(low) CD147(+) Tregs (P = 0.029) showed significant transient increase at week 8. In vitro blockade of IL-10/TGF-beta upon Mtb antigen stimulation significantly lowered the fraction of ESAT-6-specific CD4(+) CD25(high) CD127(low) Tregs at baseline (P = 0.047), while T cell proliferation and cytokine production were unaffected. Phenotypical and Mtb-specific T cell signatures may serve as markers of effective therapy, while the IL-10/ TGF-beta pathway could be a target for early inhibition to facilitate Mtb clearance. However, larger clinical studies are needed for verification before concluding.
dc.languageEN
dc.publisherBlackwell Scientific Publications
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.titleT Cell Responses and Regulation and the Impact of In Vitro IL-10 and TGF-beta Modulation During Treatment of Active Tuberculosis
dc.typeJournal article
dc.creator.authorFeruglio, Siri
dc.creator.authorKvale, Dag
dc.creator.authorDyrhol-Riise, Anne Ma
cristin.unitcode185,50,0,0
cristin.unitnameDet medisinske fakultet
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1
dc.identifier.cristin1468828
dc.identifier.bibliographiccitationinfo:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Scandinavian Journal of Immunology&rft.volume=85&rft.spage=138&rft.date=2017
dc.identifier.jtitleScandinavian Journal of Immunology
dc.identifier.volume85
dc.identifier.issue2
dc.identifier.startpage138
dc.identifier.endpage146
dc.identifier.doihttp://dx.doi.org/10.1111/sji.12511
dc.identifier.urnURN:NBN:no-58482
dc.type.documentTidsskriftartikkel
dc.type.peerreviewedPeer reviewed
dc.source.issn0300-9475
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/55708/2/Feruglio_2017_TCe.pdf
dc.type.versionPublishedVersion


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