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dc.date.accessioned2017-01-02T15:22:19Z
dc.date.available2017-01-02T15:22:19Z
dc.date.created2016-10-24T14:54:59Z
dc.date.issued2016
dc.identifier.citationSulen, Andre Gullaksen, Stein-Erik Bader, Lucius McClymont, David Skavland, Jørn Gavasso, Sonia Gjertsen, Bjørn Tore . Signaling effects of sodium hydrosulfide in healthy donor peripheral blood mononuclear cells. Pharmacological Research. 2016, 113, 216-227
dc.identifier.urihttp://hdl.handle.net/10852/53433
dc.description.abstractHydrogen sulfide (H2S) is an endogenous gasotransmitter in human physiology and inflammatory disease, however, with limited knowledge of how signal transduction pathways are involved in immune cells. To examine the effects of sulfide on relevant intracellular signaling in human peripheral blood mononuclear cells (PBMCs), we stimulated healthy donor PBMCs with sodium hydrosulfide (NaHS, 1–1000 μM) to mimic H2S stimulation, and analyzed phosphorylation of p38 mitogen activated protein kinase (MAPK) (pT180/pY182), NF-κB p65 (pS529), Akt (pS473) and CREB/ATF1 (pS133/pS63) with flow and mass cytometry. In contrast to transient effects in subsets of lymphocytes, classical monocytes demonstrated sustained phosphorylation of p38, Akt and CREB/ATF1. NaHS induced calcium dependent phosphorylation of p38, Akt and CREB, but not NF-κB, and the phosphorylation of Akt was partly dependent on p38, indicative of p38-Akt crosstalk. Attenuation of these effects by molecules targeting p38 and Hsp90 indicated Hsp90 as a possible target for H2S-induced activation of p38. These results provide a description of a NaHS-induced signal transduction pathway in human primary immune cells that may have relevance for the role of sulfides in inflammation.en_US
dc.languageEN
dc.language.isoenen_US
dc.publisherAcademic Press
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleSignaling effects of sodium hydrosulfide in healthy donor peripheral blood mononuclear cellsen_US
dc.typeJournal articleen_US
dc.creator.authorSulen, Andre
dc.creator.authorGullaksen, Stein-Erik
dc.creator.authorBader, Lucius
dc.creator.authorMcClymont, David
dc.creator.authorSkavland, Jørn
dc.creator.authorGavasso, Sonia
dc.creator.authorGjertsen, Bjørn Tore
cristin.unitcode185,29,21,1
cristin.unitnameBiO Administrasjon og kjernefasiliteter
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1
dc.identifier.cristin1394108
dc.identifier.bibliographiccitationinfo:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Pharmacological Research&rft.volume=113&rft.spage=216&rft.date=2016
dc.identifier.jtitlePharmacological Research
dc.identifier.volume113
dc.identifier.startpage216
dc.identifier.endpage227
dc.identifier.doihttp://dx.doi.org/10.1016/j.phrs.2016.08.018
dc.identifier.urnURN:NBN:no-56642
dc.type.documentTidsskriftartikkelen_US
dc.type.peerreviewedPeer reviewed
dc.source.issn1043-6618
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/53433/1/1-s2.0-S1043661816302596-main.pdf
dc.type.versionPublishedVersion


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