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dc.date.accessioned2016-09-05T10:38:24Z
dc.date.available2016-09-05T10:38:24Z
dc.date.issued2014
dc.identifier.urihttp://hdl.handle.net/10852/51916
dc.description.abstractAquaporin-4 (AQP4) water channels are concentrated in astrocytic endfoot membranes at the brain–blood and brain–cerebrospinal fluid interfaces. The mechanisms underpinning the polarized distribution of AQP4 are poorly understood. Here we tested the hypothesis that pericytes regulate AQP4 anchoring to perivascular astrocytic endfoot membranes. AQP4 immunofluorescence of brain sections obtained from novel transgenic double reporter mice expressing enhanced green fluorescent protein (eGFP) in astrocytes and Discoma Red (DsRed) in pericytes revealed strong AQP4 signal in astrocytic processes adjacent to pericytes. Quantitative immunogold analysis of C57BL/6 mice showed that the AQP4 expression was higher in endfoot membranes abutting pericytes than in those facing endothelial cells. Similar findings were made for α-syntrophin, a member of the dystrophin-associated protein complex (DAPC). The enrichment of α-syntrophin in membranes ensheathing pericytes persisted after Aqp4 gene deletion. Our data support the concept that pericytes regulate AQP4 polarization. This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.en_US
dc.language.isoenen_US
dc.relation.ispartofVindedal, Gry Fluge (2016) Molecular and functional aspects of astrocytes at the brain-blood interface. Doctoral thesis. http://urn.nb.no/URN:NBN:no-55338
dc.relation.urihttp://urn.nb.no/URN:NBN:no-55338
dc.relation.urihttp://urn.nb.no/URN:NBN:no-55338
dc.titleEvidence that pericytes regulate aquaporin-4 polarization in mouse cortical astrocytesen_US
dc.typeJournal articleen_US
dc.creator.authorGundersen, Georg Andreas
dc.creator.authorVindedal, Gry Fluge
dc.creator.authorSkare, Øivind
dc.creator.authorNagelhus, Erlend A.
dc.identifier.jtitleBrain Structure and Function
dc.identifier.volume219
dc.identifier.doihttp://dx.doi.org/10.1007/s00429-013-0629-0
dc.identifier.urnURN:NBN:no-55337
dc.type.documentTidsskriftartikkelen_US
dc.type.peerreviewedPeer reviewed
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/51916/1/10-1007-s00429-013-0629-0.pdf
dc.type.versionPublishedVersion
cristin.articleid2181


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