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dc.date.accessioned2016-08-29T13:13:17Z
dc.date.available2016-08-29T13:13:17Z
dc.date.created2016-08-19T10:59:23Z
dc.date.issued2016
dc.identifier.citationMoen, Birgitte Henjum, Kristi Måge, Ingrid Knutsen, Svein Halvor Rud, Ida Hetland, Ragna Bogen Paulsen, Jan Erik . Effect of Dietary Fibers on Cecal Microbiota and Intestinal Tumorigenesis in Azoxymethane Treated A/J Min/+ Mice. PLoS ONE. 2016, 11(5)
dc.identifier.urihttp://hdl.handle.net/10852/51756
dc.description.abstractFoods naturally high in dietary fiber are generally considered to protect against development of colorectal cancer (CRC). However, the intrinsic effect of dietary fiber on intestinal carcinogenesis is unclear. We used azoxymethane (AOM) treated A/J Min/+ mice, which developed a significantly higher tumor load in the colon than in the small intestine, to compare the effects of dietary inulin (IN), cellulose (CE) or brewers spent grain (BSG) on intestinal tumorigenesis and cecal microbiota. Each fiber was tested at two dose levels, 5% and 15% (w/w) content of the AIN-93M diet. The microbiota was investigated by next-generation sequencing of the 16S rRNA gene (V4). We found that mice fed IN had approximately 50% lower colonic tumor load than mice fed CE or BSG (p<0.001). Surprisingly, all three types of fiber caused a dose dependent increase of colonic tumor load (p<0.001). The small intestinal tumor load was not affected by the dietary fiber interventions. Mice fed IN had a lower bacterial diversity than mice fed CE or BSG. The Bacteroidetes/Firmicutes ratio was significantly (p = 0.003) different between the three fiber diets with a higher mean value in IN fed mice compared with BSG and CE. We also found a relation between microbiota and the colonic tumor load, where many of the operational taxonomic units (OTUs) related to low tumor load were significantly enriched in mice fed IN. Among the OTUs related to low tumor load were bacteria affiliated with the Bacteroides genus. These results suggest that type of dietary fiber may play a role in the development of CRC, and that the suppressive effect of IN on colonic tumorigenesis is associated with profound changes in the cecal microbiota profile.en_US
dc.languageEN
dc.language.isoenen_US
dc.publisherPublic Library of Science (PLoS)
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleEffect of Dietary Fibers on Cecal Microbiota and Intestinal Tumorigenesis in Azoxymethane Treated A/J Min/+ Miceen_US
dc.typeJournal articleen_US
dc.creator.authorMoen, Birgitte
dc.creator.authorHenjum, Kristi
dc.creator.authorMåge, Ingrid
dc.creator.authorKnutsen, Svein Halvor
dc.creator.authorRud, Ida
dc.creator.authorHetland, Ragna Bogen
dc.creator.authorPaulsen, Jan Erik
cristin.unitcode185,35,23,10
cristin.unitnameGruppe for forskningsinfrastruktur
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1
dc.identifier.cristin1374098
dc.identifier.bibliographiccitationinfo:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=PLoS ONE&rft.volume=11&rft.spage=&rft.date=2016
dc.identifier.jtitlePLoS ONE
dc.identifier.volume11
dc.identifier.issue5
dc.identifier.pagecount20
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0155402
dc.identifier.urnURN:NBN:no-55165
dc.type.documentTidsskriftartikkelen_US
dc.type.peerreviewedPeer reviewed
dc.source.issn1932-6203
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/51756/2/Moen_2016_Eff.PDF
dc.type.versionPublishedVersion
cristin.articleide0155402


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