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dc.date.accessioned2016-01-04T11:35:59Z
dc.date.available2016-01-04T11:35:59Z
dc.date.created2015-09-10T14:29:45Z
dc.date.issued2015
dc.identifier.citationEgge, Elianne Sirnæs Johannessen, Torill Vik Andersen, Tom Eikrem, Wenche Bittner, Lucie Larsen, Aud Sandaa, Ruth-Anne Edvardsen, Bente . Seasonal diversity and dynamics of haptophytes in the Skagerrak, Norway, explored by high-throughput sequencing. Molecular Ecology. 2015, 24(12), 3026-3042
dc.identifier.urihttp://hdl.handle.net/10852/48373
dc.description.abstractMicroalgae in the division Haptophyta play key roles in the marine ecosystem and in global biogeochemical processes. Despite their ecological importance, knowledge on seasonal dynamics, community composition and abundance at the species level is limited due to their small cell size and few morphological features visible under the light microscope. Here, we present unique data on haptophyte seasonal diversity and dynamics from two annual cycles, with the taxonomic resolution and sampling depth obtained with high-throughput sequencing. From outer Oslofjorden, S Norway, nano- and picoplanktonic samples were collected monthly for 2 years, and the haptophytes targeted by amplification of RNA/cDNA with Haptophyta-specific 18S rDNA V4 primers. We obtained 156 operational taxonomic units (OTUs), from c. 400.000 454 pyrosequencing reads, after rigorous bioinformatic filtering and clustering at 99.5%. Most OTUs represented uncultured and/or not yet 18S rDNA-sequenced species. Haptophyte OTU richness and community composition exhibited high temporal variation and significant yearly periodicity. Richness was highest in September–October (autumn) and lowest in April–May (spring). Some taxa were detected all year, such as Chrysochromulina simplex, Emiliania huxleyi and Phaeocystis cordata, whereas most calcifying coccolithophores only appeared from summer to early winter. We also revealed the seasonal dynamics of OTUs representing putative novel classes (clades HAP-3–5) or orders (clades D, E, F). Season, light and temperature accounted for 29% of the variation in OTU composition. Residual variation may be related to biotic factors, such as competition and viral infection. This study provides new, in-depth knowledge on seasonal diversity and dynamics of haptophytes in North Atlantic coastal waters.en_US
dc.languageEN
dc.language.isoenen_US
dc.publisherBlackwell Scientific Publications
dc.rightsAttribution-NonCommercial 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.titleSeasonal diversity and dynamics of haptophytes in the Skagerrak, Norway, explored by high-throughput sequencingen_US
dc.typeJournal articleen_US
dc.creator.authorEgge, Elianne Sirnæs
dc.creator.authorJohannessen, Torill Vik
dc.creator.authorAndersen, Tom
dc.creator.authorEikrem, Wenche
dc.creator.authorBittner, Lucie
dc.creator.authorLarsen, Aud
dc.creator.authorSandaa, Ruth-Anne
dc.creator.authorEdvardsen, Bente
cristin.unitcode185,15,29,70
cristin.unitnameSeksjon for akvatisk biologi og toksikologi
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode2
dc.identifier.cristin1263262
dc.identifier.bibliographiccitationinfo:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Molecular Ecology&rft.volume=24&rft.spage=3026&rft.date=2015
dc.identifier.jtitleMolecular Ecology
dc.identifier.volume24
dc.identifier.issue12
dc.identifier.startpage3026
dc.identifier.endpage3042
dc.identifier.doihttp://dx.doi.org/10.1111/mec.13160
dc.identifier.urnURN:NBN:no-52289
dc.subject.nviVDP::Marinbiologi: 497
dc.type.documentTidsskriftartikkelen_US
dc.type.peerreviewedPeer reviewed
dc.source.issn0962-1083
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/48373/2/Egge_et_al-2015-Molecular_Ecology.pdf
dc.type.versionPublishedVersion


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