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dc.date.accessioned2015-12-10T13:22:32Z
dc.date.available2015-12-10T13:22:32Z
dc.date.issued2013
dc.identifier.urihttp://hdl.handle.net/10852/48255
dc.description.abstractUpon stimulation of the T cell receptor, proteins involved in proximal T cell signaling gather in lipid rafts to guide external stimuli to the intracellular compartment through the formation of signaling complexes. The rearrangement of lipid rafts upon engagement of the T cell receptor is crucial to ensure a rapid and efficient signal transduction. To assess lipid raft protein composition, we here provide a qualitative mass spectrometric characterization of the Detergent- Resistant Membrane (DRM) proteome in human primary T cells, identifying a total of 425 proteins. Furthermore, we have addressed proteins solely associated with DRMs in resting T cells and proteins solely observed in DRMs upon engagement of the T cell receptor. Only a small group of proteins were found to be exclusively located to DRMs under either of these conditions, indicating that the protein composition of DRMs instimulated cells versus resting cells is remarkably stable. Classification of the 425 proteins identified in DRMs into functional categories revealed a particularly high occurrence of cytoskeletal proteins, indicating that DRMs remain attached to the underlying cortical actin cytoskeleton upon activation of T cells. © 2013 Moltu K, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.language.isoenen_US
dc.relation.ispartofMoltu, Kristine (2015) Signal network analysis of T cell activation - in human T cells from blood donors and patients with colorectal cancer. Doctoral thesis. http://urn.nb.no/URN:NBN:no-52182
dc.relation.urihttp://urn.nb.no/URN:NBN:no-52182
dc.titleA Proteomic Approach to Screening of Dynamic Changes in Detergent-Resistant Membranes from Activated Human Primary T Cellsen_US
dc.typeJournal articleen_US
dc.creator.authorMoltu, Kristine
dc.creator.authorBjørgo, Elisa
dc.creator.authorSolstad, Therese
dc.creator.authorBerge, Torunn
dc.creator.authorThiede, Bernd
dc.creator.authorTaskén, Kjetil
dc.identifier.jtitleJournal of Proteomics and Bioinformatics
dc.identifier.volume6
dc.identifier.startpage72
dc.identifier.endpage80
dc.identifier.doihttp://dx.doi.org/10.4172/jpb.1000264
dc.identifier.urnURN:NBN:no-52193
dc.type.documentTidsskriftartikkelen_US
dc.type.peerreviewedPeer reviewed
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/48255/1/moltu-et-al.pdf
dc.type.versionPublishedVersion


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