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dc.date.accessioned2015-11-27T13:16:31Z
dc.date.available2015-11-27T13:16:31Z
dc.date.created2015-09-11T14:22:31Z
dc.date.issued2015
dc.identifier.citationØynebråten, Inger Barois, Nicolas Bergeland, Trygve Kuchler, Axel Matthias Bakke, Oddmund Haraldsen, Guttorm . Oligomerized, filamentous surface presentation of RANTES/CCL5 on vascular endothelial cells. Scientific Reports. 2015, 5:9261
dc.identifier.urihttp://hdl.handle.net/10852/47973
dc.description.abstractVascular endothelial cells present luminal chemokines that arrest rolling leukocytes by activating integrins. It appears that several chemokines must form higher-order oligomers to elicit proper in vivo effects, as mutants restricted to forming dimers have lost the ability to recruit leukocytes to sites of inflammation. Here, we show for the first time that the chemokine RANTES/CCL5 binds to the surface of human endothelial cells in a regular filamentous pattern. Furthermore, the filaments bound to the surface in a heparan sulfate-dependent manner. By electron microscopy we observed labeling for RANTES on membrane projections as well as on the remaining plasma membrane. Mutant constructs of RANTES restricted either in binding to heparin, or in forming dimers or tetramers, appeared either in a granular, non-filamentous pattern or were not detectable on the cell surface. The RANTES filaments were also present after exposure to flow, suggesting that they can be present in vivo. Taken together with the lacking in vivo or in vitro effects of RANTES mutants, we suggest that the filamentous structures of RANTES may be of physiological importance in leukocyte recruitment.en_US
dc.languageEN
dc.language.isoenen_US
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleOligomerized, filamentous surface presentation of RANTES/CCL5 on vascular endothelial cellsen_US
dc.typeJournal articleen_US
dc.creator.authorØynebråten, Inger
dc.creator.authorBarois, Nicolas
dc.creator.authorBergeland, Trygve
dc.creator.authorKuchler, Axel Matthias
dc.creator.authorBakke, Oddmund
dc.creator.authorHaraldsen, Guttorm
cristin.unitcode185,53,18,12
cristin.unitnameAvdeling for immunologi og transfusjonsmedisin
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1
dc.identifier.cristin1263590
dc.identifier.bibliographiccitationinfo:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Scientific Reports&rft.volume=5:9261&rft.spage=&rft.date=2015
dc.identifier.jtitleScientific Reports
dc.identifier.volume5:9261
dc.identifier.doihttp://dx.doi.org/10.1038/srep09261
dc.identifier.urnURN:NBN:no-51945
dc.type.documentTidsskriftartikkelen_US
dc.type.peerreviewedPeer reviewed
dc.source.issn2045-2322
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/47973/2/srep09261.pdf
dc.type.versionPublishedVersion


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