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dc.date.accessioned2015-11-25T15:02:09Z
dc.date.available2015-11-25T15:02:09Z
dc.date.created2015-08-25T12:22:37Z
dc.date.issued2015
dc.identifier.citationKong, Xiang Yi Kase, Eili Tranheim Herskedal, Anette Schjalm, Camilla Damme, Markus Nesset, Cecilie Kåsi Thoresen, G. Hege Rustan, Arild Eskild, Winnie . Lack of the lysosomal membrane protein, GLMP, in mice results in metabolic dysregulation in liver. PLoS ONE. 2015, 10(6)
dc.identifier.urihttp://hdl.handle.net/10852/47884
dc.description.abstractAblation of glycosylated lysosomal membrane protein (GLMP, formerly known as NCU-G1) has been shown to cause chronic liver injury which progresses into liver fibrosis in mice. Both lysosomal dysfunction and chronic liver injury can cause metabolic dysregulation. Glmpgt/gt mice (formerly known as Ncu-g1gt/gtmice) were studied between 3 weeks and 9 months of age. Body weight gain and feed efficiency of Glmpgt/gt mice were comparable to wild type siblings, only at the age of 9 months the Glmpgt/gt siblings had significantly reduced body weight. Reduced size of epididymal fat pads was accompanied by hepatosplenomegaly in Glmpgt/gt mice. Blood analysis revealed reduced levels of blood glucose, circulating triacylglycerol and non-esterified fatty acids in Glmpgt/gt mice. Increased flux of glucose, increased de novo lipogenesis and lipid accumulation were detected in Glmpgt/gt primary hepatocytes, as well as elevated triacylglycerol levels in Glmpgt/gt liver homogenates, compared to hepatocytes and liver from wild type mice. Gene expression analysis showed an increased expression of genes involved in fatty acid uptake and lipogenesis in Glmpgt/gt liver compared to wild type. Our findings are in agreement with the metabolic alterations observed in other mouse models lacking lysosomal proteins, and with alterations characteristic for advanced chronic liver injury.en_US
dc.languageEN
dc.language.isoenen_US
dc.publisherPublic Library of Science (PLoS)
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleLack of the lysosomal membrane protein, GLMP, in mice results in metabolic dysregulation in liveren_US
dc.typeJournal articleen_US
dc.creator.authorKong, Xiang Yi
dc.creator.authorKase, Eili Tranheim
dc.creator.authorHerskedal, Anette
dc.creator.authorSchjalm, Camilla
dc.creator.authorDamme, Markus
dc.creator.authorNesset, Cecilie Kåsi
dc.creator.authorThoresen, G. Hege
dc.creator.authorRustan, Arild
dc.creator.authorEskild, Winnie
cristin.unitcode185,15,29,0
cristin.unitnameInstitutt for biovitenskap
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1
dc.identifier.cristin1259845
dc.identifier.bibliographiccitationinfo:ofi/fmt:kev:mtx:ctx&ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=PLoS ONE&rft.volume=10&rft.spage=&rft.date=2015
dc.identifier.jtitlePLoS ONE
dc.identifier.volume10
dc.identifier.issue6
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0129402
dc.identifier.urnURN:NBN:no-51897
dc.type.documentTidsskriftartikkelen_US
dc.type.peerreviewedPeer reviewed
dc.source.issn1932-6203
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/47884/2/journal.pone.0129402.pdf
dc.type.versionPublishedVersion
cristin.articleide0129402


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