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dc.contributor.authorSiddiqui, Huma
dc.contributor.authorLagesen, Karin
dc.contributor.authorNederbragt, Alexander J
dc.contributor.authorJeansson, Stig L
dc.contributor.authorJakobsen, Kjetill S
dc.date.accessioned2015-10-20T10:54:11Z
dc.date.available2015-10-20T10:54:11Z
dc.date.issued2012
dc.identifier.citationBMC Microbiology. 2012 Sep 13;12(1):205
dc.identifier.urihttp://hdl.handle.net/10852/47077
dc.description.abstractBackground Interstitial Cystitis (IC) is a chronic inflammatory condition of the bladder with unknown etiology. The aim of this study was to characterize the microbial community present in the urine from IC female patients by 454 high throughput sequencing of the 16S variable regions V1V2 and V6. The taxonomical composition, richness and diversity of the IC microbiota were determined and compared to the microbial profile of asymptomatic healthy female (HF) urine. Results The composition and distribution of bacterial sequences differed between the urine microbiota of IC patients and HFs. Reduced sequence richness and diversity were found in IC patient urine, and a significant difference in the community structure of IC urine in relation to HF urine was observed. More than 90% of the IC sequence reads were identified as belonging to the bacterial genus Lactobacillus, a marked increase compared to 60% in HF urine. Conclusion The 16S rDNA sequence data demonstrates a shift in the composition of the bacterial community in IC urine. The reduced microbial diversity and richness is accompanied by a higher abundance of the bacterial genus Lactobacillus, compared to HF urine. This study demonstrates that high throughput sequencing analysis of urine microbiota in IC patients is a powerful tool towards a better understanding of this enigmatic disease.
dc.language.isoeng
dc.rightsSiddiqui et al.; licensee BioMed Central Ltd.
dc.rightsAttribution 2.0 Generic
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/
dc.titleAlterations of microbiota in urine from women with interstitial cystitis
dc.typeJournal article
dc.date.updated2015-10-20T10:54:11Z
dc.creator.authorSiddiqui, Huma
dc.creator.authorLagesen, Karin
dc.creator.authorNederbragt, Alexander J
dc.creator.authorJeansson, Stig L
dc.creator.authorJakobsen, Kjetill S
dc.identifier.cristin996715
dc.identifier.doihttp://dx.doi.org/10.1186/1471-2180-12-205
dc.identifier.urnURN:NBN:no-51228
dc.type.documentTidsskriftartikkel
dc.type.peerreviewedPeer reviewed
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/47077/1/12866_2012_Article_1813.pdf
dc.type.versionPublishedVersion
cristin.articleid205


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