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dc.contributor.authorBrudal, Espen
dc.contributor.authorWinther-Larsen, Hanne C
dc.contributor.authorColquhoun, Duncan J
dc.contributor.authorDuodu, Samuel
dc.date.accessioned2015-10-20T10:52:45Z
dc.date.available2015-10-20T10:52:45Z
dc.date.issued2013
dc.identifier.citationBMC Research Notes. 2013 Mar 02;6(1):76
dc.identifier.urihttp://hdl.handle.net/10852/47005
dc.description.abstractBackground Reverse transcription quantitative PCR has become a powerful technique to monitor mRNA transcription in response to different environmental conditions in many bacterial species. However, correct evaluation of data requires accurate and reliable use of reference genes whose transcription does not change during the course of the experiment. In the present study exposure to different growth conditions was used to validate the transcription stability of eight reference gene candidates in three strains from two subspecies of Francisella noatunensis, a pathogen causing disease in both warm and cold water fish species. Results Relative transcription levels for genes encoding DNA gyrase (gyrA), RNA polymerase beta subunit (rpoB), DNA polymerase I (polA), cell division protein (ftsZ), outer membrane protein (fopA), riboflavin biosynthesis protein (ribC), 16S ribosomal RNA (16S rRNA) and DNA helicases (uvrD) were quantified under exponential, stationary and iron-restricted growth conditions. The suitability of selected reference genes for reliable interpretation of gene expression data was tested using the virulence-associated intracellular growth locus subunit C (iglC) gene. Conclusion Although the transcription stability of the reference genes was slightly different in the three strains studied, fopA, ftsZ and polA proved to be the most stable and suitable for normalization of gene transcription in Francisella noatunensis ssp.
dc.language.isoeng
dc.rightsBrudal et al.; licensee BioMed Central Ltd.
dc.rightsAttribution 2.0 Generic
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/
dc.titleEvaluation of reference genes for reverse transcription quantitative PCR analyses of fish-pathogenic Francisella strains exposed to different growth conditions
dc.typeJournal article
dc.date.updated2015-10-20T10:52:45Z
dc.creator.authorBrudal, Espen
dc.creator.authorWinther-Larsen, Hanne C
dc.creator.authorColquhoun, Duncan J
dc.creator.authorDuodu, Samuel
dc.identifier.doihttp://dx.doi.org/10.1186/1756-0500-6-76
dc.identifier.urnURN:NBN:no-51160
dc.type.documentTidsskriftartikkel
dc.type.peerreviewedPeer reviewed
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/47005/1/13104_2012_Article_2084.pdf
dc.type.versionPublishedVersion
cristin.articleid76


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