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dc.contributor.authorSæbøe-Larssen, Stein
dc.contributor.authorFossberg, Ellen
dc.contributor.authorGaudernack, Gustav
dc.date.accessioned2015-10-09T02:13:25Z
dc.date.available2015-10-09T02:13:25Z
dc.date.issued2006
dc.identifier.citationBMC Molecular Biology. 2006 Aug 29;7(1):26
dc.identifier.urihttp://hdl.handle.net/10852/46791
dc.description.abstractBackground Human telomerase reverse transcriptase (hTERT) is a key component for synthesis and maintenance of telomeres on chromosome ends and is required for the continued proliferation of cells. Estimation of hTERT expression therefore has broad relevance in oncology and stem cell research. Several splicing variants of hTERT have been described whose regulated expression contributes to the control of telomerase activity. Knowledge of the different hTERT mRNA isoforms and the ability to distinguish between them is an important issue when evaluating telomerase expression. Results By establishing cDNA-clone panels from lung and colon tissues, we could map hTERT clones individually for differences in DNA sequence. This made possible the identification of novel alternatively spliced sites as well as analysis of their frequency and mutual correlation in mRNA isoforms. Ten different alternatively spliced sites were detected, of which six were novel sites resulting from alternative splicing of intron 2 or 14. The majority of hTERT cDNA clones from normal and tumour lung and colon tissues encoded truncated proteins ending close after exon 2 or 6. Conclusion The increased complexity in telomerase expression revealed here has implications for our understanding of telomerase regulation and for the choice of suitable methods for addressing hTERT expression.
dc.language.isoeng
dc.rightsSæbøe-Larssen et al.; licensee BioMed Central Ltd.
dc.rightsAttribution 2.0 Generic
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/
dc.titleCharacterization of novel alternative splicing sites in human telomerase reverse transcriptase (hTERT): analysis of expression and mutual correlation in mRNA isoforms from normal and tumour tissues
dc.typeJournal article
dc.date.updated2015-10-09T02:13:26Z
dc.creator.authorSæbøe-Larssen, Stein
dc.creator.authorFossberg, Ellen
dc.creator.authorGaudernack, Gustav
dc.identifier.doihttp://dx.doi.org/10.1186/1471-2199-7-26
dc.identifier.urnURN:NBN:no-50971
dc.type.documentTidsskriftartikkel
dc.type.peerreviewedPeer reviewed
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/46791/1/12867_2006_Article_115.pdf
dc.type.versionPublishedVersion
cristin.articleid26


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