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dc.contributor.authorCekaite, Lina
dc.contributor.authorPeng, Qian
dc.contributor.authorReiner, Andrew
dc.contributor.authorShahzidi, Susan
dc.contributor.authorTveito, Siri
dc.contributor.authorFurre, Ingegerd E
dc.contributor.authorHovig, Eivind
dc.date.accessioned2015-10-09T02:12:11Z
dc.date.available2015-10-09T02:12:11Z
dc.date.issued2007
dc.identifier.citationBMC Genomics. 2007 Aug 13;8(1):273
dc.identifier.urihttp://hdl.handle.net/10852/46735
dc.description.abstractBackground Photodynamic therapy (PDT) involves systemic or topical administration of a lesion-localizing photosensitizer or its precursor, followed by irradiation of visible light to cause singlet oxygen-induced damage to the affected tissue. A number of mechanisms seem to be involved in the protective responses to PDT, including activation of transcription factors, heat shock proteins, antioxidant enzymes and apoptotic pathways. Results In this study, we address the effects of a destructive/lethal hexaminolevulinate (HAL) mediated PDT dose on the transcriptome by using transcriptional exon evidence oligo microarrays. Here, we confirm deviations in the steady state expression levels of previously identified early defence response genes and extend this to include unreported PDT inducible gene groups, most notably the metallothioneins and histones. HAL-PDT mediated stress also altered expression of genes encoded by mitochondrial DNA (mtDNA). Further, we report PDT stress induced alternative splicing. Specifically, the ATF3 alternative isoform (deltaZip2) was up-regulated, while the full-length variant was not changed by the treatment. Results were independently verified by two different technological microarray platforms. Good microarray, RT-PCR and Western immunoblotting correlation for selected genes support these findings. Conclusion Here, we report new insights into how destructive/lethal PDT alters the transcriptome not only at the transcriptional level but also at post-transcriptional level via alternative splicing.
dc.language.isoeng
dc.rightsCekaite et al; licensee BioMed Central Ltd.
dc.rightsAttribution 2.0 Generic
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/
dc.titleMapping of oxidative stress responses of human tumor cells following photodynamic therapy using hexaminolevulinate
dc.typeJournal article
dc.date.updated2015-10-09T02:12:12Z
dc.creator.authorCekaite, Lina
dc.creator.authorPeng, Qian
dc.creator.authorReiner, Andrew
dc.creator.authorShahzidi, Susan
dc.creator.authorTveito, Siri
dc.creator.authorFurre, Ingegerd E
dc.creator.authorHovig, Eivind
dc.identifier.doihttp://dx.doi.org/10.1186/1471-2164-8-273
dc.identifier.urnURN:NBN:no-50888
dc.type.documentTidsskriftartikkel
dc.type.peerreviewedPeer reviewed
dc.identifier.fulltextFulltext https://www.duo.uio.no/bitstream/handle/10852/46735/1/12864_2006_Article_986.pdf
dc.type.versionPublishedVersion
cristin.articleid273


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